Calcium activation of lipase enzyme in process of pressure split

Details Calcium activation of lipase enzyme in process of pressure split Calcium activation of lipase enzyme in process of pressure split

1996-12-19

1999-10-19

Marx, Irene

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing oxygen-containing organic compound

435198, 435135, C12P 762

Patent

active

059687924

ABSTRACT:
A process for pressure splitting glycerides into fatty acids and glycerols involving the steps of: (a) providing a glyceride feedstock; (b) providing a hydrolytic lipase enzyme; (c) providing an alkaline earth metal selected from the group consisting of calcium, magnesium, and mixtures thereof; (d) mixing components (a)-(c) in the presence of water and with agitation, at a temperature ranging from about 50 to about 60.degree. C. to form a partially hydrolyzed glyceride feedstock; (e) introducing the partially hydrolyzed glyceride feedstock into a pressure splitter; and (f) splitting the partially hydrolyzed glyceride feedstock in the pressure splitter into carboxylic acids and glycerols.

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Piazza et al., Biotechnol. Lett. (1989), 11(7), 487-492.
Van Loon, J.C., Chemical Analysis of Inorganic Constituents of Water, 1982, CRC Press, pp. 69-73.

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