Calcineurin interacting protein compositions and methods

Details Calcineurin interacting protein compositions and methods Calcineurin interacting protein compositions and methods

1994-10-24

1998-03-03

Knode, Marian C.

Drug, bio-affecting and body treating compositions

Designated organic active ingredient containing

Peptide containing doai

514 12, 514 13, 514 14, 530300, 530350, 530371, A61K 3804, A61K 3816, C07K 14395

Patent

active

057234361

ABSTRACT:
An identification and characterization of a calcineurin interacting protein effective to enhance immunosuppressive effects of calcineurin-targeted immunosuppressants by potentiating an interaction of an immunophilin with calcineurin is described herein. One embodiment of the invention is the CNI polypeptide encoded by the CNI gene of Saccharomyces cerevisiae. Polynucleotides encoding a CNI protein are also described. Also described are yeast cells carrying mutations in the CNI gene. Further, a method of identifying a small molecule immunosuppressant compound is described. The methods include the use of a cell-based two hybrid protein-protein interaction assay, wherein one of two fusion hybrid proteins in a cell contains a subunit of calcineurin, and the other of two fusion hybrid proteins contains an immunophilin.

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