Caged enzyme substrates as probes for reporter enzyme activity

Details Caged enzyme substrates as probes for reporter enzyme activity Caged enzyme substrates as probes for reporter enzyme activity

1998-12-18

1999-11-09

Leary, Louise N.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

435 4, 435968, C12Q 142, C12Q 100

Patent

active

059812079

ABSTRACT:
Caged enzyme substrates as probes for detecting reporter enzyme activity in cell-based assays are disclosed. Caged substrates for reporter gene assays are shown as compounds of Formula I or Formula II: ##STR1## wherein Z is a luminescent functionality; G is a labile group, cleavable by enzymatic action; and K is a photolytically cleavable caging group; wherein m and n are independently 0 or 1, but m and n both can not be zero. Test kits for determining reporter enzyme activity using compounds of Formula I or Formula II and photolytic cleavage to produce a compound of Formula III:

REFERENCES:
patent: 5538847 (1996-07-01), Bronstein et al.
"Handbook of Fluorescent Probes and Research Chemicals", 6.sup.th ed., Molecular Probes, Chapter 19, 448-453 (1996).
"Naphthyl Dioxetane Phosphates: Synthesis of Novel Substrates for Enzymatic Chemiluminescent Assays" J. Org. Chem. 1990, 55, 6225-6229.
"Membrane-permeable Luciferin Esters for Assay of Firefly Luciferase in Live Intact Cells" BioChem J. (1991) 276, 637-641.
During, K., J. Chrom, 618, 105-131 (1993), Month not available.
Bronstein, L., J. Biolum, Chemilum. 4, 99-111 (1989). Method not available.

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