Caffeine demethylate gene-containing DNA fragment and microbial

Details Caffeine demethylate gene-containing DNA fragment and microbial Caffeine demethylate gene-containing DNA fragment and microbial

1994-10-18

1996-08-27

Low, Christopher S. F.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing heterocyclic carbon compound having only o, n, s,...

435118, 435195, 435227, 4352523, 43525234, 4353201, 4351723, 536 232, 935 60, 930240, C12P 1718, C12N 914, C12N 978, C12N 121

Patent

active

055500412

ABSTRACT:
A DNA fragment containing a caffeine demethylase gene produced by a microorganism belonging to the genus Pseudomonas and capable of assimilating caffeine and a process for producing a 3-methyl-7-alkylxanthine comprising cultivating a novel bacterium strain of the genus Pseudomonas having been transformed with a recombinant DNA having integrated therein the above-mentioned DNA fragment in a nutrient culture medium containing a 1,3-dimethyl-7-alkylxanthine to produce a 3-methyl-7-alkylxanthine in the culture and recovering the produced 3-methyl-7-alkylxanthine from the culture are disclosed, as well as a process for producing 3-methyl-7-propylxanthine, comprising cultivating a microorganism capable of converting 1,3-dimethyl-7-propylxanthine to 3-methyl-7-propylxanthine or a mutant thereof in a nutrient culture medium containing 1,3-dimethyl-7-propylxanthine, to produce 3-methyl-7-propylxanthine in the culture and recovering the produced 3-methyl-7-propylxanthine from the culture.

REFERENCES:
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Hohnloser et al. 1980 Hoppe-Seyler's Z. Physiol. Chem., Bd 361, S. 1763-1766.
Itoh et al. 1991. J. Biochem. 110, 614-621.
Patent Abstracts of Japan, vol. 9(78), C-274, 1801, Apr. 6, 1985.
Watson et al., 1987, in: Molecular Biology of the Gene, Fourth Edition, Benjamin/Cummings Publishing Company, Inc., Menlo Park, CA, p. 313.

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