C-terminal CAT fusion protein and process of preparation by reco

Details C-terminal CAT fusion protein and process of preparation by reco C-terminal CAT fusion protein and process of preparation by reco

1988-09-23

1992-03-03

Teskin, Robin L.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 691, 43525233, 4353201, 536 27, 935 47, 935 48, 935 49, 935 51, C07H 1512, C12P 2100, C12P 2102

Patent

active

050932411

ABSTRACT:
A process for the production of a fusion protein comprising an active portion of a chloramphenicol acetyltransferase (CAT) protein and a polypeptide. The fusion protein may be purified using CAT substrate affinity chromatography. The eucaryotic polypeptide may be calcitonin or a dervative thereof such as calcitonin-glycine. Other polypeptides described include enzymes such as chymosin, prochymosin and preprochymosin, hormones such as ACTH, insulins, and growth hormones and antigenic polypetides such as foot and mouth disease virus antigenic polypetide. The fusion protein may be cleaved at a site susceptible to selective enzymic or chemical cleavage to produce free polypeptide. The fusion protein may be used as an immunogen.

REFERENCES:
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Schroder, J. et al., "Experssion Of Plant Tumor-Specific Proteins . . . ", Nucleic Acids Research 9(20):5187-5202 1981.
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Goldfarb, D. S. et al., In:Ganesan, A. T., et al., eds Molecular Cloning and Gene Regulation In Bacilli Academic Press, NY, 1982 pp. 311-324.

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