C-terminal .alpha.-amidating enzyme and process for production t

Details C-terminal .alpha.-amidating enzyme and process for production t C-terminal .alpha.-amidating enzyme and process for production t

1992-08-03

1994-11-01

Marx, Irene

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Oxidoreductase

435183, 435 681, C12N 902, C12N 900, C12P 2100

Patent

active

053607279

ABSTRACT:
The invention relates to a C-terminal .alpha.-amidating enzyme of porcine origin having the following properties: (1) the action is on a peptide or protein represented by the formula:

REFERENCES:
patent: 4708934 (1987-11-01), Gilligan et al.
patent: 4921797 (1990-05-01), Matsuo et al.
Bradbury et al. (1982) Nature, 298, 686-688.
Kizer et al. (1984) Proc. Natl. Acad. Sci. USA., 81, 3228-3232.
Stoffers et al. (1989) Proc. Natl. Acad. Sci. USA, 86, 735-739.
Eipper et al. (1985) Endocrinology, 116(6), 2497-2504.
Eipper et al. (1988) J. Biol. Chem., 263(17), 8371-8379.
Sakata et al. (1986) Biochem. Biophys. Res. Comm., 140(1), 230-236.
Eipper et al. (1987) Mol. Endorpinol. 1(11), 777-790 in Chem. Abst., 111(5), Abst. #34395.
"Mechanism of C-Terminal Amide Formation by Pituitary Enzymes", Alan F. Bradbury et al., Nature, vol. 298, (1982), pp. 686-688.
"Enzyme-catalysed Peptide Amidation", Alan F. Bradbury et al., Eur. J. Biochem., vol. 169, (1987), pp. 579-584.
"Identification in Pituitary Tissues of a Peptide .alpha.-Amidation Activity that acts on Glycine-Extended Peptides and Requires Molecular Oxygen, Copper, and Ascorbic Acid", Betty A. Eipper et al., Prod. Natl. Acad. Sci., vol. 80 (1983), pp. 5144-5148.
"Detection and Partial Characterization of an Amidating Enzyme in Skin Secretion of Xenopus laevis", Christa Mollay et al., FEBS, vol. 202, No. 2 (1986) pp. 251-254.
"Post-translational Processing in Xenopus Oocytes Includes Carboxyl-Terminal Amidation", Mary M. Bendig, The Journal of Biological Chemistry, vol. 261, No. 26 (1986), pp. 11935-11937.
"Peptide C-Terminal .alpha.-Amidating Enzyme Purified to Homogeneity from Xenopus Laevis Skin", Kensaka Mizuno et al., Biochem. Biophys. Res. Comm., vol. 137, No. 3 (1986), pp. 984-981.
"Cloning and Sequence of cDNA Encoding a Peptide C-Terminal .alpha.-Amidating Enzyme from Xenopus Laevis", Kensaka Mizuno, et al., Biochem. Biophys. Res. Comm., vol. 148, No. 2 (1987), pp. 546-552.
"Cloning of cDNA Encoding a New Peptide C-Terminal .alpha.-Amidating Activity in Rat", Junichiro Sakata et al., Biochem. Biophys. Res. Comm., vol. 140, No. 1 (1986), pp. 230-236.
"Tissue Distribution and Characterization of Peptide C-Terminal .alpha.-Amidating Activity in Rat", Junichiro Sakata et al., Biochem. Biophys. Res. Comm., vol. 140, No. 1 (1986), pp. 230-236.
"Structure of the Precursor to an Enzyme Mediating COOH-Terminal Amidation in Peptide Biosynthesis", Betty A. Eipper et al., Molecular Endocrinology, vol. 1, (1987), pp. 777-790.
"Membrane-associated Forms of Peptidylglycine .alpha.-Amidating Monooxygenase Activity in Rat Pituitary", Victor May et al., The Journal of Biological Chemistry, vol. 263, No. 16 (1988), pp. 7550-7554.
"Membrane-associated Peptidylglycine .alpha.-Amidating Monooxygenase in the Heart", Betty A. Eipper et al., The Journal of Biological Chemistry, vol. 263, No. 17 (1988), pp. 8371-8379.
"A New Facile Trinitrophenylated Substrate for Peptide .alpha.-Amidating and its Use to Characterize PAM Activity in Chromaffin Granules", Andreas G. Katopodis et al., Biochem. Biophys. Comm. Res., vol. 151, No. 1 (1988), pp. 499-505.
"Glycine-directed Peptide Amidation: Presence in Rat Brain of Two Enzymes that Convert p-Glu-His-Pro-Gly-OH into p-Glu-His-Pro-NH.sub.2 (Thyrotropin-Releasing Hormone)", J. S. Kizer et al., Proc. Natl. Acad. Sci., vol. 81 (1984), pp. 3228-3232.
"Characterization of a Substance P-Gly.sup.12 Amidating Enzyme in Human Cerebrospinal Fluid", Henning Vaeroy et al., Biochem. Biophys. Comm. Res., vol. 148, No. 1 (1987), pp. 24-30.
"Purification of a Peptidylglycine .alpha.-Amidating Enzyme from Transplantable Rat Medullary Thyroid Carcinomas", Nozer M. Mehta, Archives of Biochemistry and Biophysics, vol. 261, No. 1 (1988), pp. 44-54.
"Peptidyl-Glycine .alpha.-Amidating Mono-Oxygenase Activity Towards a Gonadotropin-Releasing Hormone C-Terminal Peptide Substrate, in Subcellular Fractions of Sheep Brain and Pituitary", Jean S. Gale et al., Biochem. J., vol. 251 (1988), pp. 251-259.
"Evidence of High Peptide .alpha.-Amidating Activity in the Pancreas from Neonatal Rats", L'Houcine Ouafik et al., Proc. Natl. Acad. Sci., vol. 84, (1987), pp. 261-264.

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