Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Patent
1995-10-18
1998-03-24
Kunz, Gary L.
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
536 268, C07H 102, C07H 1920
Patent
active
057314292
ABSTRACT:
A compound of general formula ##STR1## wherein R is methyl R' is selected from the group consisting of trityl and pixyl, and R" is H or OMe. These compounds may advantageously be employed in the synthesis of oligonucleotides by conventional methods, such as automated solid phase synthesis. Use of ethylene diamine in the cleavage and deprotection procedure substantially eliminates the formation of undesirable side products.
REFERENCES:
patent: 5348868 (1994-09-01), Reddy et al.
patent: 5428148 (1995-06-01), Reddy et al.
Miller, Paul S.; "Oligonucleoside Methylphosphonates as Antisense Reagents", Biotechnology, vol. 9, Apr. 1991, pp. 358-362.
Miller, Paul S., et al; "Solid-Phase Syntheses of Oligodeoxyribonucleoside Methylphosphonates"; Biochemistry 1986, vol. 25, pp. 5092-5097.
Miller, Paul S. et al; "Preparation of Oligodeoxyribonucleoside Methylphosphonates on a Polystyrene Support"; Nucleic Acids Research, vol. 11, No. 18, 1983.
Hogrefe, Richard I, et al; "Deprotection of Methylphosphonate Oligonucleotides Using a Novel One-Pot Procedure"; Nucleic Acids Research, 1993, vol. 21, No. 9, pp. 2031-2038.
Agrawal, Sudhir, et al; "Oligodeoxynucleoside Methylophosphonates: Synthesis and Enzymic Degradation"; Tetrahedron Letters, vol. 28, No. 31, pp. 3539-3542, 1987.
Kandimalla, Ekambar R.; et al; "Synthesis and Properties of 2'-O-Methylribonucleotide Methylphosphonate Containing Chimeric Oligonucleotides"; Nucleosides & Nucleotides, 14(3-5), pp. 1031-1035 (1995).
Vaghefi, Morteza M., et al; "A Convenient High Yield Synthesis of N4-Isobutyryl-2'-o-Methylcytidine and Its Monomer Units for Incorporation into Oligonucleotides", Nucleosides & Nucleotides, 12(10), pp. 1007-1013 (1993).
Farooqui Firdous
Hanna Naeem B.
Reddy Meda Parameswara
Beckman Instruments Inc.
Harder P. R.
Kunz Gary L.
May William H.
LandOfFree
C.sup.ac -methylphosphonamidites and methods for preparing methy does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with C.sup.ac -methylphosphonamidites and methods for preparing methy, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and C.sup.ac -methylphosphonamidites and methods for preparing methy will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2289845