Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1998-07-08
2000-02-01
Achutamurthy, Ponnathapura
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
4352523, 4352542, 4352543, 435267, 4353201, 536 232, C12N 920, C12N 1563, C12P 764, C07H 2104
Patent
active
060201800
ABSTRACT:
A DNA sequence encoding a lipase having the amino acid sequence shown in SEQ ID NO:2. The lipase has a trypsin-like catalytic triad including an active serine located in a predominantly hydrophobic, elongated binding pocket of the lipase molecule and, located in a critical position of a lipid contact zone of the lipase structure, an amino acid residue different from an aromatic amino acid residue, which amino acid residue interacts with a lipid substrate at or during hydrolysis, in which lipase variant of the amino acid residue has been replaced by an aromatic amino acid residue so as to confer to the variant an increased specific activity as compared to that of the parent lipase.
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Anant Patkar Shamkant
Borch Kim
Clausen Ib Groth
Egel-Mitani Michi
Hansen Mogens Trier
Achutamurthy Ponnathapura
Gregg, Esq. Valeta
Novo Nordisk A S
Saidha Tekchand
Zelson Esq. Steve T.
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