Butynol I esterase

Details Butynol I esterase Butynol I esterase

2011-08-30

2011-08-30

Saidha, Tekchand (Department: 1652)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing oxygen-containing organic compound

C435S135000, C435S197000

Reexamination Certificate

active

08008051

ABSTRACT:
The invention relates to novel proteins fromPseudomonas glumae, having esterase activity, in particular butynol I esterase activity, to nucleic acid sequences coding therefore, to expression cassettes, vectors and recombinant microorganisms; to methods for preparing said proteins and to the use thereof for enzymic, in particular enantioselective enzymic, ester hydrolysis or transesterification of organic esters.

REFERENCES:
Chica et al. Curr Opin Biotechnol. Aug. 2005;16(4):378-84.
Witkowski et al. Biochemistry. Sep. 7, 1999; 38(36): 11643-50.
Seffernick et al. J Bacteriol. Apr. 2001; 183 (8): 2405-10.
Arkin et al., “An algorithm for protein engineering: Simulations of recursive ensemble mutagenesis” Proc. Natl. Acad. Sci. USA 89, 7811-7815 (Aug. 1992).
Balkenhohl et al., “Optisch active Amine durch Lipase-Katalysierte Methoxyacetylierung” (Optically active amines by lipase-catalyzed methoxyacetylation) J. Prakt. Chem. 339, 381-384 (1997).
Delagrave et al., “Recursive ensemble mutagenesis” Protein Eng. 6(3), 327-331 (1993).
Faraldos et al., “Biocatalysis in Organic Synthesis. 9. Highly Enantioselective Kinetic Resolution of Secondary Alcohols Catalyzed by Acylase” Synlett 367-370 (Apr. 1997).
Gudelj et al., “Novel Rhodococcus esterases by genetic engineering” J. Mol. Cat. B: Enzymatic 5, 261-266 (1998).
Ike et al., “Solid phase synthesis of polynucleotides. VIII. Synthesis of mixed oligodeoxyribonucleotides by the phosphotriester solid phase method” Nucleic Acids Research 11(2), 477-488 (1983).
Itakura et al., “Synthesis and Use of Synthetic Oligonucleotides” Ann. Rev. Biochem. 53, 323-56 (1984).
Itakura et al., “Expression in Escherichia coli of a Chemically Synthesized Gene for the Hormone Somatostatin” Science 198, 1056-1063 (Dec. 1977).
Khalameyzer et al., “Screening, Nucleotide Sequence, and Biochemical Characterizazion of an Esterase from Pseudomonas fluorescens with High Activity towards Lactones” App. Env. Microbiol. 65(2), 477-482 (Feb. 1999).
Kuchner et al., “Directed evolution of enzyme catalysis” TIBTECH 15, 523-530 (Dec. 1997).
Manco et al., “Overexpression and properties of a new thermophilic and thermostable esterase from Bacillus acidocaldarius with sequence similarity to hormone-sensitive lipase sibfamily” Biochem. J. 332, 203-212 (1998).
Nakamura et al., “Lipase-catalyzed kinetic resolution of 3-butyn-2-ol” Tetrahedron: Asymmetry 9, 4429-4439 (1998).
Narang, “DNA Synthesis” Tetrahedron 39(1), 3-22 (1983).
Pearson et al., “Improved tools for biological sequence comparison” Proc. Natl. Acad. Sci. USA 85, 2444-2448 (Apr. 1998).
Peist et al., “Characterization of the aes Gene of Escherichia coli Encoding an Enzyme with Esterase Activity” J. Bacteriology 179(24), 7679-7686 (1997).
Reetz et al., “Superior Biocatalysts by Direct Evolution” Topics in Current Chemistry 200, 31-57 (1999).
Yang et al., “The Use of Vinyl Esters Significantly Enhanced Enantioselectivities and Reaction Rates in Lipase-Catalyzed Resolution of Arylaliphatic Carboxylic Acids” J. Org. Chem. 64, 1709-12 (1999).
Zhao et al., “Method for Optimizing Industrial Enzymes by Directed Evolution” Manual of Industrial Microbiology and Biotechnology Chapter 49, 597-604 (1999).
Butinol Esterase Mutant Experimental Data—“Expression of the truncated 335 AA butynol esterase”, Annex 1 and Annex 2.

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