Buffer system in an anti-thrombin III test

Details Buffer system in an anti-thrombin III test Buffer system in an anti-thrombin III test

1980-05-08

1981-11-24

Kepplinger, Esther M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving blood clotting factor

C12Q 156

Patent

active

043025382

ABSTRACT:
Disclosed is a procedure for conducting anti-thrombin III assays in which the conventional thrombin-anti-thrombin III treatment step is conducted at a pH of 7.9 to 8.5 maintained at the level by a buffer system comprising a buffering amount of N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid, N-2-hydroxyethylpiperazine-N'-3-propanesulfonic acid, N-tris-(hydroxymethyl)-methyl-2-amino-ethanesulfonic acid, N-tris-(hydroxymethyl)-methylglycine, or N,N-bis (2-hydroxymethyl)-glycine or a salt thereof. The compositions may also contain bacteriostatic agents.

REFERENCES:
patent: 3884896 (1975-05-01), Blomback
patent: 3947378 (1976-03-01), Babson
patent: 3985618 (1976-10-01), Innerfield
patent: 4106990 (1978-08-01), Kanges et al.
patent: 4139415 (1979-02-01), Yin et al.
Abildgaard et al, Thromb. Diath. Haemorrh. 24, 224-229 (1970).
Good et al, Biochemistry, 5(2), 467-477 (1966).
Innerfield et al, Amer. J. Clin. Path., 65, 64-68 (1976).
Kaulla et al, Amer. J. Clin. Path., 48(1), 69-80 (1967).

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