Buffer system for increasing seroconversion efficiency

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or...

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435269, 435 2, 435200, 435208, 435 696, C12Q 100, C07K 100, C12N 92440, C12P 2104

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056331304

ABSTRACT:
A method of increasing efficiency of deantigenation of blood group epitopes on erythrocytes (seroconversion) by exoglycosidases utilizing a step of performing the deantigenation in a zwitterionic buffer. The method provides a buffer system and exoglycosidase that utilizes a twenty to one hundred fold lower total enzyme mass than the prior art methods.

REFERENCES:
patent: 4330619 (1982-05-01), Goldstein
patent: 4407961 (1983-10-01), Sanders
patent: 4427777 (1984-01-01), Goldstein
patent: 4609627 (1986-09-01), Goldstein
patent: 4965203 (1990-10-01), Silbering et al.
Adams "Adenosine 5'-triphosphate determination with phosphoglycerate kinase" in Methods of Enzymatic Analysis, Academic Press, New York, NY, 539-543 (1963).
Aminoff et al., "Enzymes that destroy blood group specificity" J. Biol. Chem. 245:1659-1660 (1970).
Bradford, Anal Biochem. 72:248-254 (1976).
Bryant, in An Introduction to Immunochematology, W.B. Sanders Philadelphia, PA, pp. 280-297 (1982).
Corchete and Guerra, Phytochemistry 26:927-932 (1987).
Courtois and Petek, Methods in Enzymol. 8:565-571 (1966).
Dacie et al., "The fragility of the red blood cells: its measurements and significance" J. Path. Bact. 46:341 (1938).
Dietz et al., "Colorimetric determination of serum cholinesterase and its genetic variants by the propionyl-thiocholine-dithiobis . . . " Clin. Chem. 19:1309-1313 (1973).
Economidou et al., "Quantitative measurements concerning A and B antigen sites" Vox Sang. 12:321-328 (1967).
Feinberg and Vogelstein, Anal. Biochem. 132:6-12 (1983).
Fong et al., Transfusion, 14:551-559 (1974).
Flowers and Sharon, "Glycosidases-properties and application to the study of complex carbohydrates and cell surfaces" Adv. Enzymol. 48:29-95 (1979).
Goldstein and Swain, Phytochemistry 4:185-192 (1965).
Goldstein et al., Science 215:168-170 (1982).
Goldstein, Trans. Med. Rev. 3:206-212 (1989).
Goldstein, in Biotechnology of Blood, pp. 75-100 (1991).
Haibach et al., "Purification and characterization of a Coffea canephora .alpha.-D-galactosidase isozyme" Biochem. Biphys. Res. Comm. 191:1564-1571 (1991).
Hanahan, "Studies on transformation of E. coli with plasmids" J. Mol. bio. 166:557-580 (1983).
Harlow et al., "Immunizing animals" in Antibodies, Cold Spring Harbor Laboratory, Cold Spring harbor, NY, Chap. 5. 92-135 (1988).
Harmening, in Modern Bloodbanking and Transfusion Practices, F.A. Davis Company, Philadelphia, PA, pp. 80-82 (1989).
Harpaz et al., "Studies on B-antigenic sites of human erythrocytes by use of coffee bean .alpha.-galactosidase" Arch. Biochem. Biphys. 170:676-683 (1975).
Hata et al. "Purification and characterization of N-acetyl-.alpha.-D-galactosaminidase from Gallus domesticus" Biochem. Intl. 28:77-86 (1992).
Hobbs et al., "An ELISA for blood group specific exogylcosidases" J. Immunol. Methods. 160:261-266 (1993).
Honda et al., "Enzymic synthesis of galactooligosaccharides by trans-glycosylation with thermostable . . . " Pyconporus cinnabarinus Saga Daigaku Nogakuba Iho. 69:55-61 [n/avail].
Hoskins et al., "Mucin degradation in human colon ecosystems . . . " J. Clin. Invest. 75:944-953 (1987).
Issit, "Forces that hold red cells apart" in Applied Blood Group Serology, Montgomery (Scientific Publications, Miami, Florida) pp. 35-36 (1985).
Kitahata et al. "Synthesis of 6-O-.alpha.-D-galactosyl .alpha.-cyclodextrin . . . " Biosci. Biotechnol. Biochem. 56:1518-1519 (1992).
Kubo "Changes in the specificity of blood groups induced by enzymes from soil fungi" J. Forensic Sci. 34:96-104 (1989).
Laemmli "Cleavage of structural proteins during the assembly of the head of bacteriophage T4" Nature 227:680-685 (1970).
Landsteiner, "Uber agglutination-serscheinungen normalen menschlichen blutes" Klin. Wschr. 14:1132 (1901) [n/available].
Lenny et al., in Biotechnology of Blood (Butterworth-Heinemann, Boston, MA) pp. 75-100 (1991). [n/a -will send upon receipt].
Lenny et al., "Group B.ertyrocytes enzymatically converted to group O survive normally in A, B, and O individuals" Science 245:168-170 (1982).
Lenny et al., "Single-unit transfusions of RBC enzymatically converted from group B to group O to group A and O normal volunteers" Blood 77:1383-1388 (1991).
Levy et al. "The .alpha.-N-acetylgalactosaminadase (A-xyme) from Clostridium perfringens" Fed. Proc. 37:1601 (1978).
Levy et al., "Purification and properties of .alpha.-N-acetylgalactosaminidase from Clostridum perfringens" Biol. Chem. 255:11737-11742 (1980).
Mawhinney "Simultaneous determination of n-acetylglucosamine, N-acetylgalactosamine . . . " J. Chromatogr. 351:91-102 (1986).
Mizutani et al., "Visual disturbances, serum glycine levels and transurethral resection of the prostate" J. Urol. 144:697-699 (1990).
Mollison et al., in Blood Transfusion in Clinical Medicine. Blackwell Scientific Publications, Oxford, England, p. 593 (1987).
Moore et al., "A modified ninhydrin reagent for the photometric determination of amino acids and related compounds" J. Bio. Chem. 211:907-913 (1954).
Moran and Walker, "Molecular cloning of two novel protein kinase genes from Arabidopsis thaliana" Anal. Biochem. 132:6-12 (1993).
Overbeek et al., "Cloning and nucleotide sequence of the .alpha.-galactosidase cDNA from Cyamopis tetragonoloba (guar)" Plant Mol. Bio. 13:541-550 (1989).
Rose et al., "Direct determination of 2,3-diphosphoglycerate" Anal. Biochem. 35:177-180 (1970).
Romano and Mollison, "Red cell destruction in vivo by low concentrations of IgG anti-A" Br. J. Haematol. 29:121-127 (1987).
Schmidt in Immunobiology of the Erythrocyte, Alan R. Liss, Inc., NY, pp. 251-261 (1980).
Sharon and Fibach, "Quantitative flow cytometric analysis of ABO red cell antigens" Cytometry 12:545-549 (1991).
Sherwood et al., "Assay of plasma glycine by HPLC with electrochemical detection in patients undergoing glycine irrigation during . . . " Clinica Chimica Acta 203:275-283 (1991).
Srivastava et al., ".alpha.-L-fucosidase from bull seminal plasma: its purification and acrosome reaction promoting property" Biochem. Biophys. Res. Commun. 137:1061-1068 (1986).
Tsuji et al., "Purification and characterization of .alpha.-L-Fucosidase from Bacillus circulans grown on procine gastric mucin" J. Biochem. 107:324-330 (1990).
Watanabe and Hakomori, "Status of blood group carbohydrate chains in ontogenesis and in oncogenesis" J. Exp. Med. 144, 644-653 (1976).
Yatziv et al., "Action of .alpha.-galactosidase on glycoprotein from human B-erythrocytes" Biochem. Biophys. Res. Commun. 45:514-518 (1971).

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