Buffer shaping device

Details Buffer shaping device Buffer shaping device

1995-04-07

1996-05-21

Neibling, John

Chemistry: electrical and wave energy

Apparatus

Electrophoretic or electro-osmotic apparatus

204458, 204466, 204608, G01N 2726, G01N 27447

Patent

active

055186041

ABSTRACT:
Electrophoresis apparatus with a gel support configured and arranged to hold a gel, a buffer chamber configured and arranged to hold a buffer, with the buffer contacting each end of the gel, and a buffer shaper configured to be positioned near the gel support and in contact with the buffer. The buffer shaper is positioned nearer to one portion of the gel support than to another portion of the gel support to cause the depth of buffer between the gel support and the buffer shaper to vary along the length or width of the gel support and buffer shaper.

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W. Ansorge and S. Labeit "Field Gradients Improve Resolution on DNA Sequencing Gels" Journal of Biochemical and Biophysical Methods, 10 (3-4), (1984) 237-43.
Anders Olsson et al, "Uniformly Spaced Banding Pattern in DNA Sequencing Gels by use of Field Strength Gradient" Journal of Biochemical and Biophysical Methods, 10 (1-2), (1984), 83-90.
M. D. Biggin et al. "Buffer Gradient Gels and .sup.35 S Label as an Aid to Rapid DNA Sequence Determination" Proceeding of the National Academy of Science, USA, vol. 80 (Jul. 1983) 3963-3965.

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