Buffer gradient and temperature gradient capillary electrophores

Details Buffer gradient and temperature gradient capillary electrophores Buffer gradient and temperature gradient capillary electrophores

1991-03-25

1993-06-22

Niebling, John

Chemistry: electrical and wave energy

Processes and products

Electrostatic field or electrical discharge

204299R, G01N 2726, B01D 5702

Patent

active

052214486

ABSTRACT:
An electrophoresis instrument includes a capillary tube mounted in an air cooled cartridge. The cartridge also supports a spherical lens which is part of the optical detection apparatus. The cartridge rests in a manifold which includes the sample and buffer reservoirs. The temperature of the capillary tube is controlled by measuring the electrical resistance of the capillary tube during the electrophoresis process and then cooling or heating the cartridge by circulating temperature controlled air over the tube. The optical path associated with the instrument is a fiber optic bundle bifurcated close to dual detectors into a reference arm and a sample arm so as to provide similar reference and sample optical paths. The instrument may be used for temperature control for buffer gradient electro-phoresis and also, a neutral marker for determining electro-osmotic flow may be detected.

REFERENCES:
Anthony T. Balchunas and Michael J. Sepamiak, "Gradient Elution for Micellar Electrokinetic Capillary Chromatography" Analytical Chemistry, vol. 60, No. 7 (Apr. 1, 1988) pp. 617-621.
Michael J. Sepaniak, David F. Swaile, & A. Craig Powell "Instrumental Developments in Micellar Electrokinetic Capillary Chromatography" Journal of Chromatography, vol. 480 (Oct. 20, 1989) pp. 185-196.

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