Buffer composition and method for the electrophoretic separation

Details Buffer composition and method for the electrophoretic separation Buffer composition and method for the electrophoretic separation

1980-04-08

1981-09-29

Prescott, Arthur C.

Chemistry: electrical and wave energy

Processes and products

Electrostatic field or electrical discharge

204180S, 204299R, 23912, G01N 3316, G01N 2726

Patent

active

042921546

ABSTRACT:
A buffer composition for use in the electrophoretic separation of proteins into fractions, the buffer composition consisting essentially of Tris; an acid having the formula R.sub.1 --CO--NH--R.sub.2 --COOH where R.sub.1 is NH.sub.2 or an alkyl or aryl group, preferably NH.sub.2 , C.sub.6 H.sub.5 or C.sub.6 H.sub.4 NH.sub.2 and where R.sub.2 is an alkyl group, preferably CH.sub.2, CH.sub.2 --CH.sub.2 or CH(CH.sub.3); and a water soluble salt of the acid; the acid and salt being present in amounts and in a ratio to maintain the pH of an aqueous solution of the composition at from 8.2 to 9.0. The preferred acid is hippuric acid.

REFERENCES:
patent: 4243749 (1981-01-01), Sadeh et al.
patent: 4246084 (1981-01-01), Gurske

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