Bryodin 2 a ribosome-inactivating protein isolated from the plan

Details Bryodin 2 a ribosome-inactivating protein isolated from the plan Bryodin 2 a ribosome-inactivating protein isolated from the plan

1994-10-20

1997-01-28

Chan, Christina Y.

Drug, bio-affecting and body treating compositions

Conjugate or complex of monoclonal or polyclonal antibody,...

Conjugated to proteinaceous toxin or fragment thereof

4241781, 530300, 530350, 530370, 5303917, 536 231, 536 234, 536 235, 536 236, 514 2, 514 8, 435 691, 435 697, 435 711, 4351724, 4351713, 4353201, A61K 3944, C07K 14415, C07K 1646

Patent

active

055975690

ABSTRACT:
The present invention discloses a new ribosome-inactivating protein, bryodin 2, isolated from the plant Bryonia dioica. This ribosome-inactivating protein (RIP) is a type IRIP having a single polypeptide chain and no cellular receptor domain. Like many type I RIPs, bryodin 2 has a molecular weight of about 27,000 daltons and a pI of 9.5. Bryodin 2 differs from previously identified ribosome-inactivating protein in its amino acid composition, amino acid sequence, and toxicity in vitro and in vivo. Bryodin 2 is useful, as are other type I ribosome-inactivating proteins, as an abortifacient, immunomodulator, anti-tumor or anti-viral agent. Compositions comprising bryodin 2 as an immunoconjugate or fusion molecule are particularly useful to kill cells of a target population.

REFERENCES:
patent: 5242813 (1993-09-01), Pastan
Goldenberg Ca Cancer J Clin:44:43-64(1994).
Siegal et al. Bioconjugate Chem. 5:423-429(1994).
C. Z. Amorim et al., "Screening of the Antimalarial Activity of Plants of the Cucurbitaceae Family," Mem. Inst. Oswaldo Cruz 86:177-180, 1991.
Y. Endo et al., "RNA N-Glycosidase Activity of Ricin A-chain," J. Biol. Chem. 262:8128-8130, 1987.
P.-C. Montecucchi et al., "N-terminal sequence of some ribosome-inactivating proteins," Int. J. Peptide Protein Res. 33:263-267, 1989.
T. B. Ng et al., "Proteins with abortifacient, ribosome inactivating, immunomodulatory, antitumor and anti-AIDS activities from Cucurbitaceae plants," Gen. Pharmac. 23:575-590, 1992.
F. Stirpe et al., "Bryodin, a ribosome-inactivating protein from the roots of Bryomia dioica L. (white bryony),," Biochem. J. 240:659-665, 1986.
F. Stirpe et al., "Modification of ribosomal RNA by ribosome-inactivating proteins from plants," Nucleic Acids Research 16:1349-1357, 1988.
P. N. Friedman et al., "Antitumor Activity of the Single-Chain Immunotoxin BR96 sFv-PE40 against Established Breast and Lung Tumor Xenografts," J. Immunol. 150:3054-3061, 1993.
C. B. Siegall et al., "Characterization of Ribosome-Inactivating Proteins Isolated from Bryonia dioica and Their Utility as Carcinoma-Reactive Immunoconjugates," Bioconjugate Chem. 5:423-429, 1994.

Affiliated with

Also associated with

Rating

Bryodin 2 a ribosome-inactivating protein isolated from the plan does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Bryodin 2 a ribosome-inactivating protein isolated from the plan, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Bryodin 2 a ribosome-inactivating protein isolated from the plan will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-940111