Broth medium for detection of DNAse-positive microorganisms

Details Broth medium for detection of DNAse-positive microorganisms Broth medium for detection of DNAse-positive microorganisms

1979-05-02

1980-12-23

Warden, Robert J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

435 36, 435 38, 435253, 435254, 435255, C12Q 104

Patent

active

042411815

ABSTRACT:
A broth medium for the detection of DNAse-positive microorganisms. The medium contains conventional nutrients for growing the microorganisms, a source of essential divalent cations to insure activity of the DNAse and a biological indicator comprising DNA, toluidine blue and lambda carrageenan in sufficient quantity so that the medium turns from blue to a visually distinguishable reddish-pink or reddish-violet color in the presence of DNAse-positive microorganisms.

REFERENCES:
patent: 4035238 (1977-07-01), Meyer et al.
Jeffries et al., "Rapid Method for Determining the Activity of Microorganisms on Nucleic Acids," Journal of Bacteriology, vol. 73, p. 591, 1957.
R. V. F. Lachica et al., "Convenient Assay of Staphylococcal Nuclease by the Metachromatic Well-Agar Diffusion Technique," Applied Microbiology, vol. 24, No. 6, pp. 920-923; 1972.
Blazevic and Ederer, Principles of Biochemical Tests in Diagnostic Microbiology, John Witz and Sons, pp. 37-39, 1975.
R. V. F. Lachica et al., "Metachromatic Agar-Diffusion Methods for Detecting Staphylococcal Nuclease Activity", Applied Microbiology, vol. 21, No. 4, pp. 585-587, 1971.
Janice Schreier, "Modification of Deoxyribonuclease Test Medium for Rapid Identification of Serratin Marcescens", The American Journal of Clinical Pathology, vol. 51, No. 6, pp. 711-716, 1969.

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