Drug – bio-affecting and body treating compositions – Antigen – epitope – or other immunospecific immunoeffector – Amino acid sequence disclosed in whole or in part; or...
Patent
1997-09-03
2000-08-29
Caputa, Anthony C.
Drug, bio-affecting and body treating compositions
Antigen, epitope, or other immunospecific immunoeffector
Amino acid sequence disclosed in whole or in part; or...
4241901, 4242341, 4242461, 435 693, 530350, 536 2371, A61K 3900, A61K 3902, A61K 3907, C07K 100
Patent
active
061104647
ABSTRACT:
Disclosed are novel synthetically-modified B. thuringiensis chimeric crystal proteins having improved insecticidal activity against coleopteran, dipteran and lepidopteran insects. Also disclosed are the nucleic acid segments encoding these novel peptides. Methods of making and using these genes and proteins are disclosed as well as methods for the recombinant expression, and transformation of suitable host cells. Transformed host cells and transgenic plants expressing the modified endotoxin are also aspects of the invention.
REFERENCES:
patent: 5055294 (1991-10-01), Gilroy
patent: 5128130 (1992-07-01), Gilroy et al.
patent: 5349124 (1994-09-01), Fischhoff et al.
patent: 5380831 (1995-01-01), Adang et al.
patent: 5384253 (1995-01-01), Krzyzek et al.
patent: 5441884 (1995-08-01), Baum
patent: 5449681 (1995-09-01), Wickiser
patent: 5500365 (1996-03-01), Fischhoff et al.
patent: 5508264 (1996-04-01), Bradfisch et al.
patent: 5593881 (1997-01-01), Thompson et al.
Adang et al., "The reconstruction and expression of a Bacillus thuringienesis cryIIIA gene in protoplasts and potato plants," Plant Mol. Biol., 21:1131-1145, 1993.
Bernhard, "Studies on the delta-endotoxin of Bacillus thuringiensis var. tenebrionis," FEMS Microbiol. Letters, 33:261-265, 1986.
Herrnstadt et al., "A new strain of Bacillus thuringiensis with activity against Coleopteran insects," Bio/Technology, 4:305-308, 1986.
Hofte et al., "Structural and Functional analysis of a clones delta endotoxin of Bacillus thuringiensis berliner 1715," Eur. J. Biochem., 171:273-280, 1986.
Kller et al., "Cloning and expression of the crystal protein genes from Bacillus thuringiensis strain berliner 1715," EMBO J., 1(7):791-799, 1982.
Koziel et al., "Field performance of elite transgenic maize plants expressing an insecticidal protein derived from Bacillus thuringiensis," Bio/Technol., 11:194-200, 1993.
Krieg et al., "Bacillus thuringiensis var. tenebrionis, a new pathotype effective against larvae of Coleoptera," Z. ang. Ent., 96:500-508, 1983.
Krieg et al., "New results on Bacillus thuringiensis var. tenebrionis with special regard to its effect on the Colorado beetle (Leptinotarsa decemlineata)," Anz. Schadlingskde Pflanzenschutz Umweltschutz, 57(8):145-150, 1984.
Murray et al., "Analysis of unstable RNA transcripts of insecticidal crystal protein genes of Bacillus thuringiensis in transgenic plants and electroporated protoplasts," Plant Mol. Biol., 16:1035-1050, 1991.
Perlak et al., "Genetically improved potatoes: protection from damage by Colorado potato beetles," Plant Mol. Biol., 22:313-321, 1993.
Perlak et al., "Insect resistant cotton plants," Bio/Technol., 8:939-943, 1990.
Perlak et al., "Modification of the coding sequence enhances plant expression of insect control protein genes," Proc. Natl. Acad. Sci. USA, Biochem., 88:3324-3328, 1991.
Schnepf and Whiteley, "Cloning and expression of the Bacillus thuringiensis crystal protein gene in Escherichia coli," Proc. Natl. Acad. Sci. USA, 78(5), 2893-2897, 1981.
De Maagd et al., "Different domains of Bacillus thuringiensis .delta.-endotoxins can bind to insect midgut membrane proteins on ligand blots," Applied and Environmental Microbiology, 62(8):2753-2757, 1996.
Honee et al., "A translation fusion product of two different insecticidal crystal protein genes of Bacillus thuringiensis exhibits an enlarged insecticidal spectrum," Applied and Environmental Microbiology, 56(3):823-825, 1990.
Baum et al., "Novel Cloning Vectors for Bacillus thuringiensis," Appl. Envion. Microbiol., 56(11):3420-3428, 1990.
Bosch et al., "Recombinant Bacillus thuringiensis Crystal Proteins with New Properties: Possibilities for Resistance Management," Bio/Technology, 12:915-918, 1994.
Caramori et al., "In vivo generation of hybrids between two Bacillus thuringiensis insect-toxin-encoding genes," Gene, 98(1):37-44, 1991.
Caramori et al., "Bacillus thruingiensis kurstaki hybrid endotoxin genes generted by In Vivo recombination," ISBN 1-56081-028-9, 0(0):259-267, 1990.
Gill et al., "Identification, Isolation, and Cloning of a Bacillus thuringiensis CryIAc Toxin-binding Protein from the Midgut of the Lepidopteran Insect Heliothis virescens," J. Biol. Chem. 270(45):27277-27282, 1995.
Grochulski et al., "Bacillus thuringiensis CrylA(a) Insecticidal Toxin: Crystal Structure and Channel Formation," J. Mol. Biol., 254:447-464, 1995.
Honee et al., "The C-terminal domain of the toxic fragment of a Bacillus thuringiensis crystal protein determines receptor binding," Mol. Microbiol., 5(11):2799-2806, 1991.
Knight et al., "Molecular Cloning of an Insect Aminopeptidase N that Serves as a Receptor for Bacillus thuringiensis CryIA(c) Toxin," J. Biol. Chem., 270(30):17765-17770, 1995.
Lee et al., "Domain III Exchanges of Bacillus thuringiensis CryIA toxins affect binding to different gypsy moth midgut receptors," Biochem. Biophysical Research Communications, 216(1):306-312, 1995.
Masson et al., "The CryIA(c) Receptor Purified from Manduca sexta Displays Multiple Specificities," J. Biol. Chem., 270(35):20309-20315, 1995.
Mettus et al., "Expression of Bacillus thuringiensis .delta.-Endotoxin Genes during Vegetative Growth," Appl. Environ. Microbiol., 56(4):1128-1134, 1990.
Nakamura et al., "Construction of chimeric insecticidal proteins between the 130-kDa and 135-kDa proteins of Bacillus thuringiensis subsp. aizawai for analysis of structure-function relationship," Agric. Biol. Chem., 54(3):715-724, 1990.
Racape et al., "Properties of the pores formed by parental and chimeric Bacillus thuringiensis insecticidal toxins in planar lipid bilayer membranes," Biophysical J. 72(2) (part 2 of 2), A82, M-Pos329, 1997, ISSN:0006-3495.
Raymond et al., "Larvicidal activity of chimeric Bacillus thuringiensis protoxins," Mol. Microbiol., 4(11):1967-1973, 1990.
Rudd et al., "Domain III Substitution in Bacillus thuringiensis Delta-Endotoxin CryIA(b) Results in Superior Toxicity for Spodoptera exigua and Altered Membrane Protein Recognition," Appl. Environ. Microbiol., 62(5):1537-1543, 1996.
Rudd et al., "Different Domains of Bacillus thuringiensis .delta.-Endotixins Can Bind to Insect Midgut Membrane Proteins on Ligand Blots," Appl. Environ. Microbiol., 62(8):2753-2757, 1996.
Schnepf et al., "Specificity-determining Regions of a Lepidopteran-specific Insecticidal Protein Produced by Bacillus thuringiensis," J. Biol. Chem. 265(34):20923-20930, 1990.
Shadenkov et al., "Construction of a hybrid gene from CryIIIA and CryIA(a) .delta.-endotoxin genes of Bacillus thuringiensis and expression of its derivatives in Escherichia coli cells," Mol. Biol., 27(4):586-591, Part 2, 1993.
Thompson et al., "Structure, Function and Engineering of Bacillus thuringiensis Toxins," Genetic Engineering, 17:99-117, 1995.
Vachon et al., "Mode of action of Bacillus thuringiensis insecticidal crystal proteins: A study of chimeric toxins," FASEB Journal 10(3), A74, 429, 1996, ISSN: 0892-6638.
Gilmer Amy Jelen
Malvar Thomas
Caputa Anthony C.
Hoerner Dennis R.
Monsanto Company
Navarro Mark
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