Broad specificity DNA damage endonuclease

Details Broad specificity DNA damage endonuclease Broad specificity DNA damage endonuclease

2006-06-13

2006-06-13

Prouty, Rebecca E. (Department: 1652)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

C435S196000, C435S091100

Reexamination Certificate

active

07060455

ABSTRACT:
The present disclosure describes DNA damage endonucleases which exhibit broad specificity with respect to the types of structural aberrations in double stranded DNA. These enzymes recognize double stranded DNA with distortions in structure, wherein the distortions result from photoproducts, alkylation, intercalation, abasic sites, mismatched base pairs, insertion deletion loops, cisplatin adducts and other types of base damage (for example, uracil resulting from cytosine deamination). The UVDE (Uve1p) ofSchizosaccharomyces pombe, certain truncated forms of that UVDE (lacking from about 100 to about 250 amino acids of N-terminal sequence) and certain endonucleases fromHomo sapiens, Neurospora crassa, Bacillus subtilis, Bacillus anthracis, Methanococcus jannaschii, andDeinococcus radiodurans. The present disclosure further provides methods for cleaving double stranded DNA having structural distortions as set forth herein using the exemplified endonucleases or their stable, functional truncated derivatives.

REFERENCES:
patent: 4863874 (1989-09-01), Wassef et al.
patent: 4921757 (1990-05-01), Wheatley et al.
patent: 5077211 (1991-12-01), Yarosh
patent: 5190762 (1993-03-01), Yarosh
patent: 5225212 (1993-07-01), Martin et al.
patent: WO 99/04626 (1999-02-01), None
Hendrich B. et al, Identification and Characterization of a Family of Mammalian Methyl-CpB Binding Proteins, Mol. Cell, Biol., 1998, 18, 6538-6547, Nov. 1998.
Bellacosa A. et al, MED1, a Novel human Methyl-CpG-binding Endonuclease, Interacts with DNA Mismatch Repair Protein MLH1, Proc. Natl. Acad. Sci. USA, 1999, 96, 3969-3974, Feb. 1999.
Ford C. F. et al. Fusion Tails for the Recovery and Purification of Recombinant Proteins, protein Expression and Purification, 1991, 2, 95-107.
Kanno et al. Repair of apurinic/apirimidinic sites by UV damage endonuclease; a repair protein for UV and oxidative damage, Nucleic Acid Research, 1999, 27, 3096-3103.
Alleva and Doetsch (1998) “Characterization ofSchizosaccharomyces pombeRad2 Protein, a FEN-1 Homolog”Nucleic Acids Research26:3645-3650.
Avery et al. (1999) “Substrate Specificity of Ultraviolet DNA Endonuclease (UVDE/Uvelp) fromSchizosaccharomyces pombe” Nucleic Acids Research27:2256-2264.
Bowman et al. (1994) “A New ATP-Independent DNA Endonuclease FromSchizosaccharomyces pombeThat Recognizes Cyclobutane Pyrimidine Dimers and 6-4 Photoproducts”Nucleic Acids Research22:3026-3032.
Brash et al. (1991) “A Role for Sunlight in Skin Cancer: UV-Induced p53 Mutations in Squamous Cell Carcinoma”Proc. Natl. Acad. Sci.USA 88:10124-10128.
Chang and Lu (1991) “Base Mismatch-Specific Endonuclease Activity in Extracts fromSaccharomyces cerevisiae” Nucleic Acids Research19:4761-4766.
Davey et al. (1997) “The Fission Yeast UVDR DNA Repair Pathway Is Inducible”Nucleic Acids Research25:1002-1008.
Doetsch, P. W. (1995) “What's Old Is New: An Alternative DNA Excision Repair Pathway”Trends Biochem. Sci.20:384-386.
Doetsch et al. (1985) “T4 DNA Polymerase (3′-5′) Exonuclease, an Enzyme for the Detection and Quantitation of Stable DNA Lesions: the Ultraviolet Light Example”Nucleic Acids Research13:3285-3304.
Freyer et al. (1995) “An Alternative Eukaryotic DNA Excision Repair Pathway”Molecular and Cellular Biology15:4572-4577.
Genbank Accession No. AAA08907.
Genbank Accession No. AAD22195.
Genbank Accession No. BAA74539.
Genbank Accession No. D78571.
Genbank Accession No. U78487.
Genbank Accession No. Z49782.
Grafstrom et al. (1982) “Enzymatic Repair of Pyrimidine Dimer-Containing DNA”The Journal of Biological Chemistry257:13465-13474.
Hamilton et al. (1992) “A Eukaryotic DNA Glycosylase/Lyase Recognizing Ultraviolet Light-Induced Pyrimidine Dimers”Nature356:725-728.
Kaur et al. (1998) “Expression, Purification, and Characterization of Ultraviolet DNA Endonuclease fromSchizosaccharomyces pombe” Biochemistry37:11599-11604.
Kaur et al. (Jul. 1999), “A Uvelp-Mediated Mismatch Repair Pathway inSchizosaccharomyces pombe,” Molec. Cell. Biology19(7):4703-4710.
Kaur et al. (2000), “Ultraviolet Damage Endonuclease (Uvelp): A Structure and Strand-Specific DNA Endonuclease,”Biochemistry39(9):5788-5796.
Kim et al. (1994) “Characterization of (6-4) Photoproduct DNA Photolyase”The Journal of Biological Chemistry269:8535-8540.
Nakabeppu et al. (1982) “Purification and Characterization of Normal and Mutant Forms of T4 Endonuclease V”The Journal of Biological Chemistry257:2556-2562.
Sancar, A. (1994) “Mechanisms of DNA Excision Repair”Science266:1954-1956.
Sancar and Tang (1993) “Nucleotide Excision Repair”Photochemistry and Photobiology57:905-921.
Sancar, G.B. (1990) “DNA Photolyases: Physical Properties, Action Mechanism, and Roles in Dark Repair”Mutation Research236:147-160.
Schär and Kohli (1993) “Marker Effects of G to C Transversions on Intragenic Recombination and Mismatch Repair inSchizosaccharomyces pombe” Genetics133:825-835.
Szankasi and Smith (1995) “A Role for Exonuclease I fromS. pombein Mutation Avoidance and Mismatch Correction”Science267:1116-1169.
Takao et al. (1996) “Characterization of a UV Endonuclease Gene From the Fisson YeastSchizosaccharomyces pombeand Its Bacterial Homolog”Nucleic Acids Research24:1267-1271.
Yajima et al. (1995) “A Eukaryotic Gene Encoding an Endonuclease That Specifically Repairs DNA Damaged by Ultraviolet Light”The EMBO Journal14:2393-2399.
Yao and Kow (1997) “Further Characterization ofEscherichia coliEnconuclease V”The Journal of Biological Chemistry272:30774-30779.
Yao and Kow (1994) “Strand-Specific Cleavage of Mismatch-Containing DNA by Deoxyinosine 3′-Endonuclease fromEscherichia coli” The Journal of Biological Chemistry269:31390-31396.
Yasui and McCready (1998) “Alternative Repair Pathways for UV-Induced DNA Damage”BioEssays 20.420:291-297.
Yeh et al. (1994) “Mammalian Topoisomerase I Has Base Mismatch Nicking Activity”The Journal of Biological Chemistry269:15498-15504.
Yeh et al. (1991) “Two Nicking Enzyme Systems Specific for Mismatch-Containing DNA in Nuclear Extracts from Human Cells”The Journal of Biological Chemistry266:6480-6484.
Yonemasu et al. (1997) “Characterization of the Alternative Excision Repair Pathway of UV-Damaged DNA inSchizosaccharomyces pombe” Nucleic Acids Research25:1533-1558.
Yoon et al. (1999) “Processing of UV Damage in Vitro by FEN-1 Proteins as Part of an Alternative DNA Excision Repair Pathway”Biochemistry38(15).

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