Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Reexamination Certificate
2006-08-15
2010-12-07
Vogel, Nancy (Department: 1636)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
C435S320100
Reexamination Certificate
active
07846688
ABSTRACT:
The present invention concerns methods and compositions for the construction of a series of stable vectors for genomic library construction useful in Gram negative species. In certain embodiments, the vectors contain the pBBR1 replicon, capable of to stable replication in a broad range of Gram negative species. In various embodiments, the plasmid vectors may also contain bidirectional, rho-independent transcriptional terminators flanking the multiple cloning site, which allows for greater insert stability, and thus, greater genomic representation. Each vector may vary in its selection marker region, mobilization function, and promoter used to express insert sequences. These vectors are of use in the screening of highly representational genomic libraries in a broad variety of Gram negative species.
REFERENCES:
patent: 6709861 (2004-03-01), Mead et al.
Graupner, S. et al. “A broad-host-range expression vector series including a Ptac test plasmid, . . . ”.Biomolecular Engineering. Oct. 2000, 11-16 vol. 17 No. 1 Elsevier Science.
Product Description Document, CloneSmart DNA Cloning Kits. Lucigen Corporation, Middleton, WI. (2 pages).
Product Description Document for pBBR122 and pBHR1 Broad Host Range Vectors, MoBiTec GmbH, Gottingen, Germany. (2 pages), (Jun. 28, 1999).
ATCC Product Description Document for pBBR1Tp Vector, ATCC No. 87448. ATCC, Manassas, VA. (2 pages), (2008).
Gill Ryan
Lynch Michael
The Regents of the University of Colorado
Vogel Nancy
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