Broad host range DNA cloning system for gram-negative bacteria

Details Broad host range DNA cloning system for gram-negative bacteria Broad host range DNA cloning system for gram-negative bacteria

1984-01-09

1986-05-20

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Miscellaneous

435 68, 435 70, 435 71, 435 91, 4351723, 435253, 435 29, 435 72, 435 73, C12N 100, C12P 2100, C12P 2102, C12P 2104, C12P 1934, C12N 1500, C12N 120

Patent

active

045901635

ABSTRACT:
Novel compositions and methods for cloning DNA in a broad range of Gram-negative bacteria. Relatively small vectors are prepared substantially free of DNA sequences coding for transfer functions, while having a broad host range replication function. While the plasmid is not self-transmissible, it is transmissible by means of a helper plasmid, which desirably has a narrow host range replication function. Exogenous DNA may be inserted into the first plasmid and conjugally transferred to the target host by means of a convenient bacterial host.

REFERENCES:
Ditta et al, "Broad Host Range DNA Cloning System for Gram-Negative Bacteria: Construction of a Gene Bank of Rhizobium meliloti", Proc, Natl. Acad. Sci. USA 77:7347 (1980).
Meyer et al, "Properties of the Plasmid RK2 as Cloning Vehicle", in DNA Insertion Elements, Plasmids, and Episomes, Bukhari et al (ed.), Cold Spring Harbor Laboratory, 1977, pp. 559-566.
Buchanan et al (ed.), Bergy's Manual of Determinative Bacteriology, Williams & Wilkins Co., Baltimore, 1974, pp. 217, 262, and 428.
Thomas et al, "Regions of Broad-Host-Range Plasmid RK2 Which Are Essential for Replication and Maintenance", J. Bacteriol. 141:213 (1980).
Figurski et al, "Replication of an Origin-Containing Derivative of Plasmid RD2 Dependent on a Plasmid Function Provided in Trans", Proc. Natl. Acad. Sci. USA 76:1648 (1979).
Guiney et al, "The DNA-Protein Relaxation Complex of the Plasmid RK2: Location of the Site-Specific Nick in the Region of the Proposed origin of Transfer", Molec. Gen. Genet. 176: 183 (1979).
Meyer et al, "Unidirectional Replication of the P-Group Plasmid RK2," Biochim. Biophys. Acta 478, 109(1977).

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