Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor
Reexamination Certificate
2004-11-08
2010-02-02
Marx, Irene (Department: 1651)
Chemistry: molecular biology and microbiology
Micro-organism, per se ; compositions thereof; proces of...
Bacteria or actinomycetales; media therefor
C435S252500, C424S093100, C424S093460
Reexamination Certificate
active
07655452
ABSTRACT:
Brevibacillus choshinensisis characterized in that its extracellular proteolytic activity is extremely low and its protein secretion productivity is excellent, but it is desired that not only the extracellular proteolytic activity of the strain is further reduced but also the intracellular proteolytic activity thereof is further reduced. WhenBrevibacillus choshinensisis used as a host for the production of protein pharmaceuticals and the like, it is also desired that it does not form spores and is readily sterilized. The above problems have been solved by inactivating the sporulation-associated gene thereof and by cloning and inactivating the extracellular and intracellular protease genes thereof.
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Hanagata Hiroshi
Nishijyo Takayuki
Higeta Shoyu Co. Ltd.
Marx Irene
Oblon, Spivak McClelland, Maier & Neustadt, L.L.P.
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