Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide alters fat – fatty oil – ester-type wax – or...
Patent
1995-12-21
1997-11-18
Robinson, Douglas W.
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
The polynucleotide alters fat, fatty oil, ester-type wax, or...
800DIG26, 536 241, 4351723, 4353201, 435419, C12N 1529, C12N 1511, C12N 1582, A01H 500
Patent
active
056890532
ABSTRACT:
A Brassica sp. genomic clone containing a polygalacturonase gene promoter was isolated. This promoter directs high levels of transcription in the pollen. When the promoter was fused with the GUS gene and introduced into B. napus by Agrobacterium mediated transformation, this promoter controlled GUS expression in microspores and pollen in transgenic B. napus plants. Expression occurred between the late uninucleate microspore stage and the mature pollen grain stage. GUS activity was also identified in tapetal tissue at the binucleate microspore stage. There was no GUS expression in other tissues such as root, stem, leaf, sepal, petal or pistil. This promoter will be useful for the temporal and spatial control of endogenous gene expression in plants.
REFERENCES:
patent: 5086169 (1992-02-01), Mascarennas
patent: 5412085 (1995-05-01), Allen et al.
Laurian S. Robert, et al., Isolation and characterization of a polygalacturonase gene highly expressed in Brassica napus pollen. Plant Molecular Biology, (1993) 23:1273-1278.
Rebecca L. Allen, et al., Molecular characterization of one of the maize polygalacturonase gene gamily members which are expressed during late pollen development. The Plant Journal, (1993) 3(2):261-271.
S.J. Tebbutt, et al., Deletion analysis of tobacco pollen-specific polygalacturonase promoter, Sex Plant Reprod, (1995) 308/1-308/5.
Hai Pint Hong, et al., Transformation of Brassica napus with the GUS Reporter Gene Under the Regulation of Tissue-Specific Promoters. Abstracts VIIIth International Congress of Plant Tissue and Cell Culture, Jun. 12-17, 1994.
Gerster Jean L.
Hong Hai Ping
Robert Laurian S.
Haas Thomas
Her Majesty the Queen in right of Canada, as represented by Agri
National Research Council of Canada
Robinson Douglas W.
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