Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Patent
1996-02-06
1999-07-06
Achutamurthy, Ponnathapura
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
536 236, 435 691, 435410, 435419, 4353201, 800200, 800205, C07H 2100, C12P 2100, A01H 300, C12N 514
Patent
active
059199199
ABSTRACT:
A Brassica sp. genomic clone containing a gene promoter, which directs expression in the tapetum, was isolated. When the promoter was fused with the GUS gene and introduced into B. napus by Agrobacterium mediated transformation, this promoter controlled GUS expression in the tapetum in transgenic B. napus plants. Expression was observed from bud length 2-3 mm, which corresponds to the uninucleate microspore stage, peaked at 4-5 mm which corresponds to the binucleate microspore stage and declined to no detectable level at the open flower stage. There was no GUS expression in other tissues such as root, stem, leaf, sepal, petal or pistil. This promoter will be useful for the temporal and spatial control of gene expression in plants.
REFERENCES:
patent: 5401836 (1995-03-01), Baszczynski et al.
patent: 5612472 (1997-03-01), Wilson et al.
patent: 5659026 (1997-08-01), Baszczynski et al.
patent: 5689053 (1997-11-01), Robert et al.
Abstract, "Transformation of Brassica Napus with the GUS Reporter Gene Under the Regulation of Tissue-Specific Promoters", Hong, H.P., et al., Abstracts VIIIth International Congress of Plant Tissue and Cell Culture, Firenze, Jun. 12-17, 1994.
Hong Hai Ping
Robert Laurian
Achutamurthy Ponnathapura
Bui Phuong T.
Her Majesty the Queen in right of Canada, as represented by Agri
National Research Council of Canada
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