Brassica sp. gene promoter highly expressed during stigma develo

Details Brassica sp. gene promoter highly expressed during stigma develo Brassica sp. gene promoter highly expressed during stigma develo

1996-03-18

1999-05-25

Robinson, Douglas W.

Multicellular living organisms and unmodified parts thereof and

Method of introducing a polynucleotide molecule into or...

The polynucleotide alters fat, fatty oil, ester-type wax, or...

800DIG15, 536 236, 536 241, 4351723, 4353201, C12N 1529, C12N 1582, A01H 400, A01H 500

Patent

active

059070838

ABSTRACT:
A Brassica sp. genomic clone G 363 containing a gene promoter, which directs expression in the stigma, was isolated. Based on microbombardment studies of Brassica napus flowers with the gene Pis 63/Gus fusion, the Pis 63 promoter was shown to direct GUS expression to the stigma. This promoter will be useful for the temporal and spatial control of gene expression in plants.

REFERENCES:
Roger Lewin. When does homology mean something else? Science. vol. 237, 1570, Sep. 1987.
Reeck et al. "Homology" in proteins and nucleic acids: A terminology muddle and a way out of it. Cell. vol. 50, 667, Aug. 1987.
Thorsness et al. A Brassica S-locus gene promoter targets toxic gene expression and cell death to the pistil and pollen of transgenic Nicotiana. Developmental Biology 143: 173-184 (1991).
Robert et al. Molecular analysis of two Brassica napus genes expressed in the stigma. Plant Molecular Biology 26: 1217-1222, 1994.
Hackett, R.M. et al. (1992). "A Brassica S-locus related gene promoter directs expression in both pollen and pistil of tobacco", The Plant Journal 2:613-617.
Dzelzkalns, V.A. et al. (1993). "Distinct cis-Acting Elements Direct Pistil-Specific and Pollen-Specific Activity of the Brassica S Locus Glycoprotein Gene Promoter", The Plant Cell 5:855-863.
Goldman, M.H.S. et al. (1994). "Female sterile tobacco plants are produced by stigma-specific cell ablation", The EMBO Journal 13:2976-2984.
"Molecular Analysis of two Brassica Napus genes expressed in the stigma" Laurian S. Robert, et al., Plant Molecular Biology, 26:1217-1222, 1994.

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