Drug – bio-affecting and body treating compositions – Antigen – epitope – or other immunospecific immunoeffector – Virus or component thereof
Reexamination Certificate
1999-01-15
2004-10-19
Scheiner, Laurie (Department: 1648)
Drug, bio-affecting and body treating compositions
Antigen, epitope, or other immunospecific immunoeffector
Virus or component thereof
C424S186100, C424S204100
Reexamination Certificate
active
06805867
ABSTRACT:
BACKGROUND OF THE INVENTION
Bovine rotavirus (BRV) is a major cause of diarrhea in young calves. Infectious virions of BRV typically have a core protein designated VP2, an inner capsid protein designated VP6, and two outer capsid proteins designated VP4 and VP7. The BRV strains which infect both young and old animals are classified serologically into different groups and subgroups primarily on the basis of epitopes present on VP6. At present there are three BRV groups, designated A, B, and C, which are known to infect calves and adult cattle. The BRV groups are further classified into G serotypes on the basis of epitopes on VP7 and into P serotypes on the basis of epitopes present on VP4. This classification scheme provides important information about the strains of BRV infecting young calves.
Unfortunately, conventional serotyping methods do not permit separate analysis of rotavirus G and P types, and fail to detect subtypes or monotypes of a particular G serotype, limiting their usefulness for field samples.
Recently, a method for genotyping field isolates has been developed which is based on nucleic acid hybridization of probes to viral RNA. The genotyping permits the diagnosis of the strain infecting a particular animal. However the method is limited to diagnosing those strains for which certain genes sequences are known since the production of specific the probes requires knowledge of the gene sequence.
It would be desirable to know the sequences of genes of additional bovine rotavirus strains so as to design probes useful in the diagnosis of bovine rotavirus.
SUMMARY OF THE INVENTION
The present invention relates to novel genes of new field isolates of bovine rotavirus which permit the genotyping and thus the diagnosis of such new strains. The present invention provides the genes encoding the following BRV proteins: for group A, the VP4 and VP7 proteins of the Indiana (IND) strain, and the VP7 protein of the 2292B strain; for group B, the VP7 protein of the WD653 strain; for group C, the VP4 and VP7 proteins of the Shintoku strain. The genes are useful for producing nucleic acid probes which are complementary to the VP7 and VP4 genes. Such probes are useful for detecting the presence of group A,B, and C BRV in fecal samples from diarrheic calves and for determining the serotype of the BRV field isolates. The genes are also useful for producing partial length nucleic acid probes which are complementary to hypervariable regions of the VP4 and VP7 genes.
The present invention also relates to partially purified VP2, VP4, VP6 and VP7 proteins of the IND strain and VP4 and VP7 of the 2292B strain, the partially purified VP7 protein of the WD653 strain, and partially purified VP2, VP4 and VP7 proteins of the Shintoku strain. The present invention also relates to recombinant virus-like particles (VLPs) which comprise one or more of the VP2, VP4, VP6, and VP7 proteins of the BRV strains IND, 2292B, CR, WD653, and Shintoku.
REFERENCES:
patent: 5298244 (1994-03-01), Redmond et al.
Tsunemitsu, H., et al., 1996, “Sequence comparison of the VP7 gene encoding the outer capsid glycoprotein among animal and human group C rotaviruses”, Arch. Virol. 141:705-713.*
Tsunemitsu, H., et al., 1992, “Evidence of serologic diversity within Group C rotaviruses”, J. Clin. Microbiol. 30(11):3009-3012.*
“Characterization of Field Strains of Group A bovine Rotaviruses by Using a Polymerase Chain Reaction-Generated G and P Type-Specific cDNA Probes” by Parwani, et al.,Journal of Clinical Microbiology, vol. 31, No. 8, Aug. 1993, pp. 2010-2015.
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“Isotype-specific antibody responses to rotavirus and virus proteins in cows inoculated with subunit vaccines composed of recombinant SA11 rotavirus core-like particles (CLP) or virus-like particles (VLP)” by Fernandez, et al., Thu Jul. 4 14:00:02 1996: JVAC: 415: tx1.
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“A survey of G6 and G10 serotypes of group A bovine rotaviruses from diarrheic beef and dairy calves using monoclonal antibodies in ELISA” by Lucchelli,J Vet Diagn Invest6:175-181 (1994).
“Molecular and serologic characterization of a group A bovine rotavirus with a short genome pattern” by Parwani, et al,J Vet Diagn Invest, 7:255-261 (1995).
“Comparative Nucleotide and Deduced Amino Acid Sequence Analysis of VP7 gene of G6 IND and G10 2292B field group A BRV strains” by Parwani, et al., Genbank Accession No. U15000, Feb. 16, 1996.
“Sequence Analysis of VP3 and VP4 genes of a bovine group C rotavirus: molecular evidence for a new P type” by Jiang, et al., Genbank Accession No. BRU26551, Jun. 5, 1996.
Chang Kyeong-Ok
Gadfield Kathy
Kim Wonyong
Parwani Anil
Saif Linda J.
Calfee Halter & Griswold LLP
Parkin Jeffrey S.
Scheiner Laurie
The Ohio State University Research Foundation
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