Chemistry: analytical and immunological testing – Involving an insoluble carrier for immobilizing immunochemicals – Carrier is organic
Patent
1982-03-09
1984-07-17
Nucker, Christine M.
Chemistry: analytical and immunological testing
Involving an insoluble carrier for immobilizing immunochemicals
Carrier is organic
436534, 436542, 436800, 436804, 436812, 260112B, 435 7, G01N 3354, G01N 3356, C07G 700
Patent
active
044606940
ABSTRACT:
A novel purification scheme is described for obtaining two novel and useful forms of bovine glycoprotein (BGP) from bovine erythrocytes, each of which acts as an antigen in testing for the presence of the heterophile antibodies of human infectious mononucleosis.
The first, or partially purified, BGP is obtained from crude BGP and contains about 10% by weight of a complex glycoplipid. It forms a single band upon gel electrophoresis at pH 7.0 under specified conditions.
The second, or homogeneous, BGP is obtained by removing essentially all of the complex glycolipid. It forms substantially a single band upon gel electrophoresis at pH 7.0 under specified conditions.
Both forms may be used to detect or quantify hemagglutination inhibition of a test sample (and hence to determine the presence or extent to mononucleosis infection) in a glass slide test wherein the partially purified or homogeneous BGP is carried on latex or synthetic resin beads.
The homogeneous BGP is also useful in an immunoassay, preferably a radioimmunoassay, for heterophile antibodies characteristic of infectious mononucleosis.
REFERENCES:
patent: 3426123 (1969-02-01), Hoff
patent: 3639558 (1972-02-01), Catzmas et al.
patent: 3708572 (1973-01-01), Petoom et al.
patent: 3826821 (1974-07-01), Zichis
patent: 3840655 (1974-10-01), Lerner
patent: 3857931 (1974-12-01), Hager
patent: 3864467 (1975-02-01), Leikolin
patent: 3882225 (1975-05-01), Patel et al.
patent: 3959456 (1976-05-01), Zichis
patent: 4046723 (1977-09-01), Dorman
patent: 4228148 (1980-10-01), Zichis et al.
Merrick et al., Chem. Abstracts, vol. 87, (1977), abstract #198965f.
Fletcher, J. Immunol., vol. 117, (1976); 722-729.
Fletcher et al., Chem. Abstracts, vol. 81, (1974), Abstract #76159b.
Fletcher et al., Chem. Abstracts, vol. 87, (1977), Abstract #49104x.
Callahan, Chem. Abstracts, vol. 86, (1977), Abstract #3370g.
Marchesi, V. T. et al. Ann. Rev. Biochem. 45, 667 (1976), The Red Cell Membrane.
Paul, J. R. et al. Amer. J. Med. Sci. 183, 90 (1932), The Presence of Heterophile Antibodies in Infectious Mononucleosis.
Davidsohn, I. et al. Amer. J. Clin. Pathol. 5, 455 (1935), The Nature of Heterophilic Antibodies in Infectious Mononucleosis.
Bailey, G. H. et al. J. Clin. Invest. 14,228 (1935), Hemolytic Antibodies for Sheep and Ox Erythrocytes in Infectious Mononucleosis.
Evans, A. S. et al. J. Infect. Dis. 132,546 (1975), A Prospective Evaluation of Heterophile and EB Virus-specific IgM Antibody Tests.
Fletcher, M. A. et al. J. Immunol. 107,842 (1971), Immunochemical Studies of Infectious Mononucleosis.
Levey, B. A. et al. J. Clin. Microbio. 11,256 (1980), Latex Test for Serodiagnosis of Infectious Mononucleosis.
Lee, C. L. et al. Amer. J. Clin. Pathol. 49,12 (1968), Horse Agglutinins in Infectious Mononucleosis.
Editorial, Brit. Med. J. 280,1153 (1980), Tests for Infectious Mononucleosis.
Fraker, P. J. et al. Biochem, Biophys. Res. Commun. 80,849 (1978), Protein & Cell Membrane Iodinations with a Sparingly Soluble Chloramide.
Svennerholm, L. Biochem. Biophys. Acta 24,604 (1957), Quantitative Estimation of Sialic Acids.
Fletcher, M. A. et al. J. Immunol. Methods. 14,51 (1977), Immunochemical Studies of Infectious Mononucleosis.
Dejter-Juszynski, M. et al. Eur. J. Biochem. 83,363 (1978), Blood-Group ABH-Specific Macroglycolipids of Human Erythrocytes: Isolation in High Yield from a Crude Membrane Glycoprotein Fraction
Nucker Christine M.
University of Miami
LandOfFree
Bovine glycoproteins and use in diagnosing infectious mononucleo does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Bovine glycoproteins and use in diagnosing infectious mononucleo, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Bovine glycoproteins and use in diagnosing infectious mononucleo will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1491531