Drug – bio-affecting and body treating compositions – Extract – body fluid – or cellular material of undetermined... – Waste or feces
Reexamination Certificate
1999-07-07
2001-06-19
Witz, Jean C. (Department: 1651)
Drug, bio-affecting and body treating compositions
Extract, body fluid, or cellular material of undetermined...
Waste or feces
Reexamination Certificate
active
06248367
ABSTRACT:
TECHNICAL FIELD
The present invention relates to bovine excretion extracts and a process for preparing the same. More specifically, the present invention relates to bovine excretion extracts having not only anticancer activity by potent inhibition of cell proliferation and by anti-oxidation but also anti-inflammatory activity by potent inhibition of platelet aggregation, which are useful as an anticancer agent or adjuvant, or an anti-inflammatory agent, and the process for preparing the same.
BACKGROUND ART
The term “cancer” is a generic name of malignant tumors. It means the state wherein part of cells begin abnormal proliferation and continue the proliferation to invade neighboring normal tissues and to break down cell functions. Cells abnormally proliferate because they get out of the control of their regulatory mechanisms because they are genetically mutated.
Anticancer agents which have been developed hitherto for treating various cancers are broadly classified into chemical anticancer agents and immunological anticancer agents (modification substances of biological reactions). The chemical agents display their effect because proliferative potency of cancer cells is different from that of normal cells. The immunological agents expel cancer cells by reviving immunocompetence in an organism.
Chemical anticancer agents are usually toxic to the cells and display their effect based on the principle that cancer cells have stronger proliferative potency than normal cells. That is, if chemical anticancer agents which suppress cell proliferation are administered to a living body, cancer cells having stronger proliferative potency will be influenced more severely than normal cells.
Chemical anticancer agents which have been developed to the present time can be classified into five types: alkylating agents, metabolic antagonists, antibiotic anticancer agents, botanical anticancer agents, etc. Alkylating agents are medicines which display anticancer effect by alkylation of hydrogen atoms of nucleic acids or proteins. Metabolic antagonists are antagonists against purine or pyrimidine bases and display anticancer effect by inhibition of DNA replication. Antibiotic and botanical anticancer agents are derived from microorganisms and plants, respectively. Other examples of anticancer agents are L-asparaginase or cisplatin (platinum compound)
As described previously, chemical anticancer agents by inhibiting cell proliferation derived from microorganisms or plants have been already reported. However, anticancer agents derived from animals have not been reported in the art.
From old times, animal excretion has been used for treating various diseases in a folk remedy. That is, human excretion has been traditionally used for treating a cut or a bruise, and bovine excretion has been used for detoxification or treating a burn. In addition, it has been known that wounded animals take bovine excretion. As above-described, although animal excretion has been traditionally used for medicines, scientific studies thereon have not been performed to the present time. Moreover, it has not been expected that animal excretion can be used as an anticancer agent or adjuvant, or an anti-inflammatory agent.
DISCLOSURE OF THE INVENTION
Accordingly, the present inventors repeated scientific studies to find out physiological activity of bovine excretion. As a result, they discovered that bovine excretion extracts prepared by a process according to the present invention have anticancer activity by potent inhibition of cell proliferation and by anti-oxidation, and anti-inflammatory activity by potent inhibition of platelet aggregation, and finally, completed the present invention.
An object of the present invention is to provide novel bovine excretion extracts having anticancer activity by inhibition of cell proliferation and by anti-oxidation, and anti-inflammatory activity by inhibition of platelet aggregation, which are useful as an anticancer agent or adjuvant, or an anti-inflammatory agent.
Another object of the present invention is to provide a process for preparing the bovine excretion extracts.
The present invention provides bovine excretion extracts having anticancer and anti-inflammatory activity prepared by a process comprising the step of extracting bovine excretion with a polar organic solvent selected from the group consisting of lower alcohol having 1 to 4 carbon atom(s), lower ketone of the formula: R
1
COR
2
, wherein R
1
and R
2
independently of one another represent alkyl having 1 to 3 carbon atom(s), acetonitrile, water and mixtures thereof. In a preferable embodiment, the present bovine excretion extracts are prepared by re-extracting the extracts with a nonpolar organic solvent selected from the group consisting of water-saturated butanol, ethyl acetate and ethyl ether. And the bovine excretion is preferably excrement of cattle or buffaloes.
The present invention also provides an anticancer agent comprising the bovine excretion extracts as active ingredient. In a preferable embodiment, the anticancer agent is orally administered with a unit dose of 100 to 500 mg, about 3 times a day.
The present invention also provides an anti-inflammatory agent comprising the bovine excretion extracts as active ingredient. In a preferable embodiment, the anti-inflammatory agent is orally administered with a unit dose of 100 to 500 mg, about 3 times a day.
The present invention provides a process for preparing bovine excretion extracts having anticancer and anti-inflammatory activity comprising the step of extracting bovine excretion with a polar solvent selected from the group consisting of lower alcohol having 1 to 4 carbon atom(s), lower ketone of the formula: R
1
COR
2
, wherein R
1
and R
2
independently of one another represent alkyl having 1 to 3 carbon atom(s), acetonitrile, water and mixtures thereof. In a preferable embodiment, the process further comprises the step of re-extracting the extracts with a nonpolar organic solvent selected from the group consisting of water-saturated butanol, ethyl acetate and ethyl ether.
REFERENCES:
Hosono et al., Jpn J Zootech Sci 56(3): 274-278 (1985). Abstract.*
Chemical Abstracts, vol. 113, No. 11, p. 81, abstract 91505 (1990).
Database WPI on EPOQUE, week 9503, London: Derwent Publications Ltd., AN 95-019 182, PN: JP 06-305 970 A.
Database WPI on EPOQUE, week 8501, London: Derwent Publications Ltd., AN 85-004170, PN: JP 59-205 320 A.
Database WPI on EPOQUE, week 9716, London: Derwent Publications Ltd., AN 97-175677, PN: JP 09-040 657 A.
Chung Jin Ho
Kim Bok Deuk
Lee Seung Ki
Park Jeong Hill
Park Man Ki
Corless Peter F.
Dike Bronstein Roberts & Cushman
Kim Bok Deuk
Witz Jean C.
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