Bovine embryo medium

Chemistry: molecular biology and microbiology – Spore forming or isolating process

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Details

4352402, 43524025, 4352403, C12N 500, C12N 502

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active

050968220

ABSTRACT:
The present invention is directed to a bovine embryo culture medium. The medium includes a culture solution containing the nutritional substances necessary to support a bovine embryo. One of the nutritional substances is hemicalcium lactate in an amount effective to maintain the health and viability of the bovine embryo. The culture medium is a preferred nuclear transfer medium.

REFERENCES:
patent: 4994384 (1991-02-01), Prather et al.
patent: 5057420 (1991-10-01), Massey
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Bondioli, K. R. et al., 1990, "Production of Identical Bovine Offspring by Nuclear Transfer," Theriogenology, vol. 33, No. 1, pp. 165-174.
Marek, D. E. et al., 1990, "Nuclear Transplantation in the Bovine: Effect of Donor Embryo Age on Subsequent Embryo Production," Theriogenology, vol. 33, No. 1, p. 283.
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Zhang, X. and D. T. Armstrong (1990, "Presence of Amino Acids and Insulin in a Chemically Defined Medium Improves Development of 8-Cell Rat Embryos In Vitro and Subsequent Implantation In Vivo", Biol. Reprod., 42, 662-668).
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Parrish, J. J. et al., (1988, "Capacitation of Bovine Sperm by Heparin," Biol. Reprod. 38, 1171-1180).
Leibfried, M. L. and B. D. Bavister, 1982, "Effect of Epinephrine and Hypotaurine on in vitro Fertilization in the Golden Hamster," J. Reprod. Fertil., 66, 87-93.
Seshagiri, P. B. and B. D. Bavister, 1989, "Glucose Inhibits Development of Hamster 8-cell Embryos In Vitro," Biol. Reprod., 40, 599-606.
Prather, R. S. et al., 1987, "Nuclear Transfer in the Bovine Embryo: Assessment of the Donor Nuclei and Recipient Cytoplasm," Biol. Reprod., 37, 859-866.

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