Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1995-04-24
1998-03-24
Spiegel, Carol A.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 792, 436516, 436528, 436531, 436532, G01N 3353
Patent
active
057311617
ABSTRACT:
An assay for the identification of neutralizing botulinum antibodies in sera is provided which includes the steps of separating non-sodium dodecyl sulfate, non-trypsinized complex botulinum toxin in an arylamide gel by electrophoresis, the separation occurring on a basis of botulinum toxin protein size and charge. Thereafter, the separated protein is electrophoretically transferred onto a solid support, the transferred separated protein being bound to the solid support at spaced apart sites. Remaining sites on the solid support not occupied by bound protein are blocked and the solid support and bound protein are contacted with a sera sample containing an antibody directed against botulinum toxin. The contacted solid support is then exposed to a second antibody capable of reacting to produce an insoluble colored substrate of intensity relative to a quantity of antibodies present. Finally, the second antibody is reacted to produce the insoluble colored substrate which is visually identified.
REFERENCES:
patent: 4452901 (1984-06-01), Gordon et al.
patent: 5356772 (1994-10-01), Chan et al.
patent: 5562907 (1996-10-01), Arnon
Schmidt et al., "Purification of Type E Botulinum Neurotoxin by High-Performance Ion Exchange Chromatography," Analytical Biochem, 156:213-219, Jul. 1986.
De Maio, A. "Protein blotting and immunoblotting using nitrocellulose membranes." in: Dunbar, B. S., Protein Blotting:a practical approach (New York, Oxford University Press, 1994). pp. 11-14.
L. Siegel, "Human Immune Response to a Botulinum Pentavalent (ABCDE) Toxoid Determined by a Neutralization Test and by an Enzyme-Linked Immunosorbent Assay," J. Clin. Micro. 26(11):2351-2356, Nov. 1988.
O. Bjerrum, "Native Immunoblotting of Membrane Proteins Separated in Presence of Non-Ionic Detergent" in CRC Hanbook of Immunblotting of Proteins, vol. II CRC Press, Inc 1988; pp. 49-63.
Aoki Kei Roger
Spanoyannis Athena Faye
Allergan Inc.
Hackler Walter A.
Spiegel Carol A.
LandOfFree
Botulinum toxin antibody detection assay does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Botulinum toxin antibody detection assay, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Botulinum toxin antibody detection assay will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2287933