Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing hydrocarbon
Reexamination Certificate
2011-07-26
2011-07-26
Robinson, Hope (Department: 1652)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing hydrocarbon
C435S069100, C435S183000, C435S320100, C530S350000, C530S300000, C536S023200
Reexamination Certificate
active
07985568
ABSTRACT:
This application relates to the functional identification and characterization of a nucleic acid molecule encoding a triterpene synthase, in particular botryococcene synthase. Also described are host cells comprising the nucleic acid molecules of this invention, proteins encoded by the nucleic acid molecules and methods for using the nucleic acid molecules, transformed hosts and encoded proteins to produce high levels of triterpene hydrocarbons.
REFERENCES:
Wells, Biochemistry, vol. 29, pp. 8509-8517, 1990.
Seffernick et al. (J. Bacteriology, vol. 183, pp. 2405-2410, 2001).
Blagg BSJ, Jarstfer MB, Rogers DH, Poulter CD (2002) Recombinant squalene synthase. A mechanism for the rearrangement of presqualene diphosphate to squalene. Journal of the American Chemical Society 124: 8846-8853.
Jarstfer MB, Zhang DL, Poulter CD (2002) Recombinant squalene synthase. Synthesis of non-head-to-tail isoprenoids in the absence of NADPH. Journal of the American Chemical Society 124: 8834-8845.
Poulter CD (1990) Biosynthesis of Non-Head-to-Tail Terpenes—Formation of 1′-1 and 1′-3 Linkages. Accounts of Chemical Research 23: 70-77.
Chappell Joe
Devarenne Tim
Niehaus Tom
Okada Shigeru
Crowell & Moring LLP
Robinson Hope
University of Kentucky Research Foundation
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