Boron silicates, aluminum silicates, and phosphosilicates for pu

Chemistry of inorganic compounds – Boron or compound thereof – Oxygen containing

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423304, 423325, C01B 33113, C01B 3326

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active

055253199

ABSTRACT:
Boron silicates, phosphosilicates and aluminum silicates useful as binding surfaces for DNA purification. These compounds allow DNA to be bound and eluted under native conditions (i.e., in the absence of chaotropes or alcohols) using only water, low salt buffers or physiological buffers. Methods for preparation of the compounds and methods for purifying DNA using the compounds are also provided.

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Mazurin et al. Handbook of Glass Data, Part A, Silica Glass and Binary Silicate Glasses, Physical Sciences Data 15, Elsevier, New York (1983), pp. 539, 543, and 594-595.
C. W. Chen, et al. "Recovery of DNA Segments from Agarose Gels" Anal. Biochem. 101, 339-341 (1980).
R. Boom, et al. "Rapid and Simple Method for Purification of Nucleic Acids" J. Clin. Microbiol. 28, 495-503 (1990).
B. Vogelstein, et al. "Preparative and analytical purification of DNA from agarose" Proc. Natl. Acad. Sci. 76, 615-619 (1979).
A. N. Murachkevich, et al. "Manufacture of Silicon Phosphate" Chemical Abstracts vol. 114, No. 4, Jan. 28, 1991, Abstract No. 26721h.
T. Sato, et al. "Manufacture of crystalline Laminar Sodium Borosilicate" Chemical Abstracts vol. 117, No. 26, Dec. 28, 1992, Abstract No. 254348j.

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