Bone resorption assay based on a peptide liberated during collag

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435 792, 435 793, 435 794, 435971, 435975, 436536, 436538, 436540, 436808, 530323, 5303881, 5303891, 5303911, 5303913, G01N 3353, G01N 33535

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055388531

ABSTRACT:
An antibody raised to type I collagen carboxyterminal cross-linked telopeptide isolated from decalcified human or animal bone, may be used in an assay to determine the concentration of liberated carboxyterminal telopeptide region of the type I collagen molecule in a sample. When the sample is a body fluid such as serum or urine the degradation product measured is resistant to further degradation, since it contains a multivalent intermolecular cross-link, and can thus be found in the body fluid. The assay may be used to assess the degradation of type I collagen, the major organic constituent of bone matrix.

REFERENCES:
patent: 4973666 (1990-11-01), Eyre
Kuhn, "Chemical Properties of Collagen", in Immunochemistry of the Extracellular Matrix, H. Furthmayr, Ed., CRC Press, Inc., Boca Raton, Fla. (1982), pp. 1-29.
Hartmann et al., "Radioimmunoassay of Type I Collagen that Mainly Detects Degradation Products in Serum: Application to Patients with Liver Disease", Clin. Chem. vol. 36, No. 3, pp. 421-426 (1990).
Wu et al., "Fine Powdering Exposes the Mineral-Protected Collagen of Bone to Protease Digestion", Calcif. Tissue Int., vol. 42, pp. 243-247, (1988).
Risteli et al., "Bone Resorption Assay Based on a Peptide Liberated During Type I Collagen Degradation", J. Bone Min. Res., 6 (Supp.1), SZ51(Abstract 670), 1991.
Ericksen et al., "Cross-Linked Carboxyterminal Telopeptide of Type I Collagen in Serum (S-ICTP): A New Bone Resorption Marker", J. Bone Min Res., 6(Suppl), 5243 (Abstract 637), 1991.

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