Bone metabolism related protein and gene thereof

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

Reexamination Certificate

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C435S069100, C435S320100, C435S325000, C435S375000, C435S377000, C530S399000, C536S023510

Reexamination Certificate

active

07070946

ABSTRACT:
The present invention is directed to a novel method of detecting a function or activity of a polypeptide which is related to bone metabolism, in particular, differentiation (maturation) of osteoblast or morphological change (retraction), specifically relating to a polypeptide which comprises an amino acid sequence shown by SEQ ID NO: 2 or SEQ ID NO: 4, an amino acid sequence in which one or several amino acids are deleted, substituted or added in the amino acid sequence shown by SEQ ID NO: 2 or SEQ ID NO: 4, or a polypeptide encoded by a nucleic acid which is capable of hybridizing under stringent condition with a nucleic acid comprising a nucleotide sequence shown by SEQ ID NO: 1 or SEQ ID NO: 3, or a complement sequence thereof.

REFERENCES:
patent: 5194596 (1993-03-01), Tischer et al.
patent: 2000-139467 (2000-05-01), None
patent: WO 01/12662 (2001-02-01), None
Wells, Additivity of Mutational Effects in Proteins. Biochemistry 29:8509-8517 (1990).
Karp, What we do not know about sequence analysis and sequence databases. Bioinformatics 14:753-754 (1998).

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