Chemistry: molecular biology and microbiology – Carrier-bound or immobilized enzyme or microbial cell;... – Enzyme or microbial cell is immobilized on or in an organic...
Patent
1997-11-26
2000-08-01
Naff, David M.
Chemistry: molecular biology and microbiology
Carrier-bound or immobilized enzyme or microbial cell;...
Enzyme or microbial cell is immobilized on or in an organic...
424 9463, 424 9464, 424423, 435180, 514 56, 530402, 530815, 530816, 536 21, C12N 1106, C08B 3710, C07K 100, A61K 3848
Patent
active
060965258
ABSTRACT:
A bio-active material such as heparin, urokinase or streptokinase is bonded via a hydrophilic spacer to a functionality on the surface of a hydrophobic, bio-compatible polymeric substrate to provide a coating of the bio-active material on the surface. The substrate surface is preferably the surface of an implantable medical device such as a vascular graft. An amine and/or hydroxyl functionality on the substrate surface is reacted with an isocyanate group at an end of the hydrophilic spacer to bond the spacer to the substrate, and the bio-active material is reacted with an isocyanate group at another end of the spacer to bond the bio-active material to the spacer. Polymeric substrates include expanded polytetrafluoroethylene and polyethylene terephthalate, and the hydroxyl and/or amine functionality may be provided on the substrate surface by plasma glow discharge. A preferred spacer is an isocyanate end-blocked poly(ethylene oxide). Reaction of the functionality of the substrate with the isocyanate group of the spacer may be catalyzed with dibutyltindilaurate, and reaction of the bio-active material with the isocyanate group of the spacer may be catalyzed with 1-(3 -dimethylaminopropyl)-3-ethylcarbodiimide.
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Piao et al., Heparin Immobilization by Surface Amplification, ASAIO Journal, No. 3, Jul./Sep. 1992, pp. 638-643.
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Meadox Medicals Inc.
Naff David M.
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