Organic compounds -- part of the class 532-570 series – Organic compounds – Heavy metal containing
Patent
1986-04-30
1989-06-06
Raymond, Richard L.
Organic compounds -- part of the class 532-570 series
Organic compounds
Heavy metal containing
548110, 556402, 556421, C07F 710
Patent
active
048373486
ABSTRACT:
The invention relates to the functionalization of particulate bonded phase chromatographic supports prepared by silanization of silica gel or controlled pore glass and containing pendant primary alkyl amine groups. Functionalization results from the activation of the amines by reaction with N,N'-carbonyldiimidazole (CDI), or a related azolide, in anhydrous organic solvent, followed by derivatization of the support. Derivatization results from reaction of the activated support with a functionalizing reagent consisting of a primary or secondary, alkyl or aryl amine in organic solvent, or from an aqueous solution of the amine or its salt. A urea linkage results through which the functionalizing reagent is covalently attached to the support. Derivatization can result from the addition of an excess of a single reagent, or as a consequence of the sequential addition of two or more functionalizing reagents. Chromatographic supports preparation in this manner yield materials suitable for affinity, covalent, ion exchange and hydrophobic interaction chromatography of biomolecules as well as for the preparation of immobilized reagents.
REFERENCES:
patent: 2907782 (1959-10-01), Pike
patent: 4316041 (1982-02-01), Totten et al.
patent: 4435567 (1984-03-01), Lugosi et al.
Stolowitz Mark L.
Taketomo Amy G.
Cole Stanley Z.
Fisher Gerald M.
Raymond Richard L.
Schnapf David
Varian Associates Inc.
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