Blotting technique for membrane assay

Details Blotting technique for membrane assay Blotting technique for membrane assay

1989-08-22

1992-10-13

Johnston, Jill A.

Chemistry: analytical and immunological testing

Including sample preparation

Liberation or purification of sample or separation of...

2041801, 422101, 436518, 436807, G01N 3300

Patent

active

051550490

ABSTRACT:
A technique for passaging liquid through a membrane putatively containing, in its interstices, at least one substance for which detection is desired, comprises positioning donor and acceptor bibulous matrices onto either surface of the membrane and squeezing the resulting sandwich. This technique permits the application of small volumes of reagents or wash to the membranes and the facile recovery of the waste. The subject membranes are obtained, for example, by blotting developed electrophoresis gels or directly by their use as supports for specific binding assays.

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patent: 4427415 (1984-01-01), Cleveland
patent: 4632901 (1986-12-01), Valkirs et al.
patent: 4704255 (1987-11-01), Jolley
patent: 4826759 (1989-05-01), Guire et al.
"Molecular Cloning, A Laboratory Manuel" T. Maniatis et al. (Cold Spring Harbor Laboratory: New York) 1982 pp. 383-386.
Hoefer Scientific Instruments, Publication, Transfer Electrophoresis, Hybrid-Ease advertisement, pp. 60-61.

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