Blot overlay assay using colloidal metal particles

Details Blot overlay assay using colloidal metal particles Blot overlay assay using colloidal metal particles

1987-10-27

1988-10-04

Nucker, Christine M.

Chemistry: analytical and immunological testing

Involving an insoluble carrier for immobilizing immunochemicals

436525, 436528, 436531, 436805, 436541, 436807, 436824, G01N 33553, G01N 33538

Patent

active

047756365

ABSTRACT:
A method for detecting and/or determining an agglomerate formed by the reaction between a specific binding agent and the corresponding acceptor substance according to the general methodology of blot overlay assays by using colloidal metal particles labelled components which may be visualized as a coloured signal at the surface of the blotting medium characteristric for the colloidal metal particles used or quantitatively determined following art-known spectrophotometric procedures such as densitometry.

REFERENCES:
patent: 4115198 (1978-09-01), Coughlin et al.
patent: 4487839 (1984-12-01), Kamentsky
Gershoniet Al., Analyt. Biochem. 131 (1983) 1-15.
Leuvering et al., J. Immunoassay, 1 (1980) 77-91.

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