Chemistry: molecular biology and microbiology – Maintaining blood or sperm in a physiologically active state...
Patent
1991-12-06
1995-05-23
Chan, Christina Y.
Chemistry: molecular biology and microbiology
Maintaining blood or sperm in a physiologically active state...
435 7021, 43524027, 53038823, 53038825, 4241401, A01N 102, C12P 2108, C07K 1528, A61K 39395
Patent
active
054181291
ABSTRACT:
A method of producing monoclonal antibodies that bind to tumor-associated gangliosides, the method comprising: (1) immunizing a host with tumor cells; (2) boosting the host with a suspension comprising a mixture of tumor cell membrane and at least one purified lactonized tumor-associated ganglioside; (3) boosting the host with an immunogen comprising a lactone of a tumor associated ganglioside adsorbed on or incorporated into a carrier; (4) fusing immunized cells from the host with myeloma cells to form hybridoma cells; (5) selecting hybridoma cells that produce antibody that binds to the ganglioside of step (3); (6) culturing the selected hybridoma cells; and (7) recovering the antibody.
REFERENCES:
patent: 4904596 (1990-02-01), Hakomori
Ritz J. et al, The Lancet, 2:60-63, Jul. 10, 1982.
Clausen Henrik
Hakomori Sen-itiroh
Muroi Kazuo
Nojiri Hisao
Nudelman Edward
Chan Christina Y.
Jichi Medical School
Loring Susan A.
The Biomembrane Institute
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