Blood testing method

Details Blood testing method Blood testing method

2007-10-02

2007-10-02

Wallenhorst, Maureen M. (Department: 1743)

Chemistry: analytical and immunological testing

Including sample preparation

Liberation or purification of sample or separation of...

C436S063000, C436S174000, C436S175000, C436S045000, C435S002000

Reexamination Certificate

active

10676107

ABSTRACT:
A blood test method including (a) centrifuging a mammalian blood sample into a plasma and blood cells; (b) removing buffy coats from the blood cells to obtain red blood cells containing solutes confined therewithin; (c) washing the red blood cells with a buffered physiological saline solution and isolating the washed blood cells; (d) mixing the washed red blood cells with a buffered physiological saline solution to obtain a suspended liquid; (e) centrifuging the suspended liquid to remove a supernatant and to obtain a red blood layer; (f) mixing the red blood layer with a hypertonic solution and maintaining the resulting suspension at a temperature of 25 to 40° C. for a period of time sufficient for the solutes confined in the red blood cells to penetrate into the hypertonic solution; (g) centrifuging the suspension to obtain a supernatant containing the solutes; and (h) measuring the supernatant for at least one factor selected from a glucose concentration, a pyruvic acid concentration, a lactic acid concentration and an oxidation-reduction potential.

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Patent Abstracts of Japan, vol. 13, No. 40 (P-820), Jan. 30, 1989.
Lester, E., “The Clinical Value of Glycated Hemoglobin and Glycated Plasma Proteins”, Annals of Clinical Biochemistry, vol. 26, No. 3, 1989, pp. 213-219.

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