Chemistry: analytical and immunological testing – Metal or metal containing – Present in biological fluids
Patent
1993-11-08
1995-06-27
Czaja, Donald E.
Chemistry: analytical and immunological testing
Metal or metal containing
Present in biological fluids
422 681, 436 73, 436 77, 436166, 436171, 436182, G01N 3320
Patent
active
054279534
ABSTRACT:
A method for testing blood samples for determining lead content therein. The method utilizes filter collection paper that is spotted with a blood sample; the blood sample spotted thereon is allowed to dry. The dried blood sample on the paper is punched out in a predefined manner to obtain a uniform volume equivalent to a predefined volume of whole blood. A dilute aqueous acid reagent containing a surfactant wetting agent is utilized to remove the part of the blood sample containing the lead. The filter collection paper retains the hemoglobin and blood proteins thus yielding a protein free solution that is suitable for direct analysis of the trace lead elements. The dried blood sample may be easily stored and economically transported to testing laboratories where the dried blood sample may be eluted into a hemoglobin and protein free solution that is stable for weeks under proper storage conditions. The method allows easy, economical and accurate testing of blood samples using standard analytical chemical measurement of trace elements such as lead.
REFERENCES:
patent: 3708228 (1973-01-01), Delves
patent: 4920057 (1990-04-01), Castaneda
patent: 5019516 (1991-05-01), Wiese
patent: 5217594 (1993-06-01), Henkens et al.
patent: 5252489 (1993-10-01), Macri
Czaja Donald E.
The Detroit Medical Center
Tran Lien
LandOfFree
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