Blood fractionation improvement

Details Blood fractionation improvement Blood fractionation improvement

1982-03-29

1984-08-14

Tufariello, T. M.

Chemistry: electrical and wave energy

Processes and products

Electrostatic field or electrical discharge

424 88, 424101, B01D 5702, C25B 700, A61K 3900

Patent

active

044655746

ABSTRACT:
The extraction of Factor VIII, the antihaemophilic factor in blood, is difficult due to its instability and the presence of impurities. An initial Factor VIII containing aqueous solution such as blood plasma is purified by subjecting a Factor VIII containing aqueous migrant solution to continuous flow electrophoresis wherein flow takes place in an annular separation chamber and is stabilized by means of an angular velocity gradient; and collecting a separated Factor VIII component.
In order to separate the Factor VIII from albumin and firbrinogen, while obtaining good recoveries of Factor VIII (e.g. .about.60%), the migrant solution is prepared by precipitating Factor VIII from the initial solution using ethanol, and removing and redissolving the precipitate in an aqueous medium and adjusting the pH to be within the range of 7.5 to 8.6, preferably 8.3 to 8.6.

REFERENCES:
patent: 3616453 (1971-10-01), Philpot
patent: 3844926 (1974-10-01), Smyth et al.
patent: 4149957 (1979-04-01), Gibson et al.
patent: 4246085 (1981-01-01), Mattock
patent: 4250008 (1981-02-01), Mattock
Chem. Abstracts, vol. 88, Nov. 10, 1977, 55060s.
Chem. Abstracts, vol. 94, Nov. 6, 1980, 36335t.
Chem. Abstracts, vol. 52, No. 21, 11-10-58, Col. 18751(g).
Chem. Abstracts, vol. 54, No. 1, 1-10-60, Col. 672(b).

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