Chemistry: analytical and immunological testing – Composition for standardization – calibration – simulation,... – Preservative – buffer – anticoagulant or diluent
Patent
1987-12-16
1990-11-06
Wax, Robert A.
Chemistry: analytical and immunological testing
Composition for standardization, calibration, simulation,...
Preservative, buffer, anticoagulant or diluent
436 17, 436 8, 2524081, G01N 3100
Patent
active
049686291
ABSTRACT:
A universal blood diluent for use in hematology analysis is the subject of this invention. The diluent consists of an aqueous solution of 0.4-1.5% by weight of either Na.sub.2 SO.sub.4 or NaNO.sub.3 or a combination of the two. Also included is 0.1-0.4% by weight of a salt of the formula XH.sub.2 PO.sub.4 where X is either Na or K and 0.1-2.4% by weight of a salt of the formula X.sub.2 HPO where X is Na or K. The phosphate salts may be hydrous or anhydrous. A mixture of Na and K phosphate salts may also been employed. The salts should be present in a ratio of from 1:1 to 1:6 (by weight), XH.sub.2 PO.sub.4 :X.sub.2 HPO.sub.4. 0.1 to 1% by weight of one or both of NaCl and KCl is also included. The Na.sub.2 SO.sub.4 and NaNO.sub.3 should be present in a ratio of at least 1:1 (by weight) relative to the chloride salt. The pH of the diluent should be within the range of 6.0-8.0 and it should have an osmotic strength of 200-400 milliosmoles. The diluent may be used in either automated or semi-automated analytical equipment. The use of an inorganic buffer offers numerous advantages over prior art organic buffers including cost an better performance. The diluent allows for leukocyte analysis as well as maintaining erythrocyte morphology until a lysing reagent is introduced.
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Hematology Marketing Associates, Inc.
Wallen T. J.
Wax Robert A.
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