Blood-culture flask with integrated subculture

Details Blood-culture flask with integrated subculture Blood-culture flask with integrated subculture

1985-08-26

1987-07-07

Cohan, Alan

Chemistry: molecular biology and microbiology

Apparatus

Bioreactor

215227, 215276, 215277, C12M 124

Patent

active

046787534

ABSTRACT:
A blood-culture flask with an integrated subculture. It comprises a flask proper that can be filled with a liquid nutrient solution and has a neck with an outside thread, a support that can be coated with a solid culture medium, a resilient stopper with an edge that projects at one end, and a screw cap. The screw cap consists of a bottom that is open above and below and has an inside thread and of a top that is open below. The inside thread is dimensioned so that it can be screwed down over the outside thread on the neck of the flask so that the upper edge of the thread is below the upper edge of the neck when screwed on. The resilient stopper is positioned inside the bottom of the screw cap so that the bottom surface of its resilient projecting edge comes to rest against the offset above the thread when not screwed on and its lower section is dimensioned so that it can be introduced tight into the neck of the flask. The support has two surface that can be coated with a solid culture medium on opposite sides, is fastened to the bottom of the resilient stopper, and extends in use only over part of the height of the flask, and the top of the screw cap is designed so that in assembled state it forces the resilient projecting edge of the stopper tightly against the upper edge of the neck of the flask.

REFERENCES:
patent: 3499568 (1970-03-01), Riera
patent: 3589983 (1971-06-01), Holderith
patent: 3651926 (1972-03-01), Elfast
patent: 3871545 (1975-03-01), Bereziat
patent: 4230231 (1980-10-01), Burnett
Castaneda-Ruiz, M., 7/1947, "Castaneda Blutkulturflasche; Kulturtechnik", 1 pg.
Review Article, H. K. Baird et al, "Industrial Applications of Some New Microbial Polysaccharides", Biotechnology, Nov. 1983, pp. 778-783.
Difco Manual, Baco, MacConkey, Agar, "Dehydrated Culture Media", pp. 131-133, 9th ed., 8/1977.

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