Blood cell sorting methods and systems

Details Blood cell sorting methods and systems Blood cell sorting methods and systems

2011-01-11

2011-01-11

Wallenhorst, Maureen M (Department: 1797)

Chemistry: analytical and immunological testing

Composition for standardization, calibration, simulation,...

Particle count or volume standard or control

C436S008000, C436S018000, C436S063000, C436S149000, C436S150000, C436S174000, C436S175000, C436S177000, C436S178000, C435S002000, C252S408100, C422S073000, C210S695000

Reexamination Certificate

active

07867765

ABSTRACT:
The invention relates to methods of isolating white blood cells (WBCs) from a sample, e.g., whole blood, using magnetic particles that specifically bind to WBCs and a series of specific steps and conditions. The methods can include one or more of decreasing the viscosity of the sample prior to WBC isolation, agitating the sample at specified frequencies, and/or using a sample container arranged such that all of the sample is placed in close proximity (e.g., within 5, 2, 1, or 0.5 mm) to the source of the magnetic field. The new methods provide for isolation of WBC preparations with high yield, purity, and viability. The methods are designed for compatibility with automation protocols for rapid processing of multiple samples.

REFERENCES:
patent: 3700555 (1972-10-01), Widmark et al.
patent: 3709791 (1973-01-01), Lichtenstein
patent: 4664796 (1987-05-01), Graham et al.
patent: 4910148 (1990-03-01), Sorensen et al.
patent: 5155044 (1992-10-01), Ledis et al.
patent: 5260192 (1993-11-01), Russell et al.
patent: 5411863 (1995-05-01), Miltenyi
patent: 6074884 (2000-06-01), Siiman et al.
patent: 6194204 (2001-02-01), Crawford et al.
patent: 6361749 (2002-03-01), Terstappen et al.
patent: 6417011 (2002-07-01), Miltenyi
patent: 6576428 (2003-06-01), Assenmacher et al.
patent: 6586259 (2003-07-01), Mahan et al.
patent: WO 87/01607 (1987-03-01), None
patent: WO 99/19730 (1999-04-01), None
patent: 2004/013155 (2004-02-01), None
Cox et al., “Automating Procedures for Processing, Cryopreservation, Storage, and Manipulation of Human Peripheral Blood Mononuclear Cells,” JALA, 9:16-23 (2004).
Sorenson et al., “Distribution of blood viscosity values and biochemical correlates in healthy adults,” Clin. Chem., 42:1189-95 (1996).
Dyanl Biotech, “CD8 Positive Isolation Kit,” Jul. 2, 2003.
Bruno et al, “Development of an immunomagnetic assay system for rapid detection of bacteria and leukocytes in body fluids,” J. Mol. Recog., 9:474-479 (1996).
Brinchmann et al, “Direct immunomagnetic quantification of lymphocyte subsets in blood,” Clin. Exp. Immunol., 71:182-186 (1988).
Miltenyi Biotec, “Whole Blood CD8 MicroBeads,” 140-000-684.03 (2005).
Rasmussen et al, “A new method for detachment of Dynabeads from positively selected B lymphocytes,” J. Immunol. Meth., 146:195-202 (1992).
Safarik et al, “Use of magnetic techniques for the isolation of cells,” J. Chromatogr. B Biomed. Sci. Appl., 722:33-53 (1999).
Supplemental Search Report issued in EP 06 84 8249, PCT/US2006049437, dated Jul. 16, 2009.

Affiliated with

Also associated with

Rating

Blood cell sorting methods and systems does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Blood cell sorting methods and systems, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Blood cell sorting methods and systems will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2622722