Blocked-polymerase polynucleotide immunoassay method and kit

Details Blocked-polymerase polynucleotide immunoassay method and kit Blocked-polymerase polynucleotide immunoassay method and kit

1992-12-24

1998-12-15

Jones, W. Gary

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 71, 435 911, 435 912, 435810, 436501, 536 221, 536 231, 536 241, 536 243, 536 2431, 536 2432, 536 2433, 935 78, 935 88, C12Q 168, C07H 2104, G01N 3353

Patent

active

058494782

ABSTRACT:
An immunoassay method for detecting an analyte in a liquid sample is disclosed. The method includes first contacting the sample with a polynucleotide assay reagent composed of a analyte and an attached polynucleotide containing an initiation region adjacent the site of attachment to the analyte. The sample is reacted with a polymerase and nucleotide triphosphates, to determine the amount of immunocomplex formed between the analyte and the analyte under conditions effective to copy the polynucleotide only if its initiation region is not blocked. The assay mixture is then assayed for the presence of phosphate or pyrophosphate. An immunoassay kit for detecting an analyte in a liquid sample is also disclosed.

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Stratogene Catalog (published 1988 by Stratogene Cloning Systems, La Jolla, CA) p. 26.
Sano, T., et al., "Immuno-PCR: Very Sensitive Antigen Detection by Means of Specific Antibody-DNA Conjugates," Science 258:120-122 (1992).

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