Organic compounds -- part of the class 532-570 series – Organic compounds – Carboxylic acid esters
Patent
1988-02-19
1989-08-08
Kight, John
Organic compounds -- part of the class 532-570 series
Organic compounds
Carboxylic acid esters
560160, 560169, 560171, 546184, 546290, C07D20952, C07C10384, A61K 3140, A61K 31195
Patent
active
048554863
ABSTRACT:
A method is provided for quantitatively monitoring the deprotection and coupling reactions employed in the solid phase synthesis of peptides. The method entails synthesizing a peptide on a support matrix that has a first marker associated therewith. The amino acids employed in the peptide synthesis procedure have a second marker attached thereto, which can be the blocking group used for the amino acid. After the coupling or deprotection step a portion of the support matrix is processed to release first and second identifers from the first and second markers, respectively. The completeness of the coupling or deprotection step can be determined by comparing the relative amounts of the detected first and second identifiers. Novel compositions of matter are used in or produced during this method, including support matrixes having pyrolyzable markers attached thereto.
REFERENCES:
patent: 3334969 (1967-08-01), Catravas
patent: 3346486 (1967-10-01), Winter et al.
patent: 3518240 (1970-06-01), Tilak et al.
patent: 3578164 (1971-05-01), Johnson
patent: 3717436 (1973-02-01), Penabsi et al.
patent: 3814732 (1974-06-01), Wang
patent: 3925267 (1978-12-01), Coupek et al.
patent: 4076913 (1978-02-01), Walker et al.
patent: 4105789 (1978-08-01), Ondetti et al.
patent: 4283504 (1981-08-01), Campbell et al.
patent: 4515920 (1985-05-01), Erickson
patent: 4582875 (1986-04-01), Ngo
patent: 4623484 (1986-11-01), Carpino et al.
patent: 4624962 (1986-11-01), Henning et al.
Tam et al., Synthesis, 12: 955-957 (1979).
Merrifield, J. American Chem. Soc., 85: 2149-2154.
Meienhofer, "Large Scale Peptide Synthesis: A Review", 18th European Symposium, Stockholm, Sweden.
Esko et al., Acta Chem. Scand., 22: 3342-3344 (1986).
Brunfeldt et al., Acta Chem. Scand., 23: 2906-2907 (1969).
Dorman, Tetrahedron Letters, 2519-2521 (1969).
Kaiser et al., Anal. Biochem., 34: 595-598 (1970).
Felix, Anal. Bio. Chem., 52: 377-381 (1973).
Gisin, Helv. Chim. Acta, 56: 1476-1482 (1973).
Frankhauser et al., Helv. Chim. Acta, 57: 271-277 (1974).
Christensen, Acta Chemica Scandinavia B, 33: 763-766 (1979).
Kaiser et al., Anal. Chim. Acta, 118: 149-151 (1980).
Sarin et al., Anal. Biochem., 117: 147-157 (1981).
March, Advanced Organic Chemistry: Reactions Mechanisms, and Structure, pp. 746-780, McGraw-Hill, N.Y., N.Y. (1968).
DePuy et al., Chem. Rev., 60: 431-457 (1960).
Mitchell et al., J. Amer. Chem. Soc., 98: 7357-7362 (1976).
Kalbag et al., Peptides: Structure and Function, Proceedings of the 9th American Peptide Symposium, 269-272 (1985).
Kalbag et al., Abstract, Ninth American Peptide Symposium.
Kight John
Nutter Nathan M.
LandOfFree
Blocked, marked amino acids does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Blocked, marked amino acids, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Blocked, marked amino acids will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-907113