Bisulfite conversion of DNA

Details Bisulfite conversion of DNA Bisulfite conversion of DNA

2011-06-28

2011-06-28

Martinell, James (Department: 1634)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S091100, C435S091200

Reexamination Certificate

active

07968295

ABSTRACT:
The present invention provides an improved method for the bisulfite conversion of DNA, and facilitates the analysis of cytosine methylation of genomic DNA. Novel combinations of denaturing solvents, new reaction conditions and new purification methods provide surprisingly efficacious methods for bisulfite conversion of DNA relative to prior art methods. The converted DNA may subsequently be analyzed by many different methods.

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Grunau et al., “Bisulfite genomic sequencing: systematic investigation of critical experimental parameters”,Nucleic Acids Res., 29(13):E65-5 (2001).
Paulin et al., “Urea improves efficiency of bisulphite-mediated sequencing of 5′-methylcytosine in genomic DNA”,Nucleic Acids Res., 26(21):5009-10 (1998).

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