Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Patent
1993-11-15
1995-07-11
Ceperley, Mary E.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
435 405, 435 4052, 435 1, 435 723, 435960, 435962, 436 18, 436826, G01N 33543
Patent
active
054320569
ABSTRACT:
The present invention provides a bisulfite-based tissue fixative that improves morphology, antigen retention, and antigenicity. The fixative is suitable for use with sections of frozen tissue, cytology preparations (including touch preparations and blood smears) and other specimens used for immunohistochemical analysis. The fixative comprises an acidic buffer and a bisulfite salt in an effective amount for tissue fixation. In a preferred embodiment, the tissue fixative comprises a bisulfite salt in a concentration of from about 0.01 to about 0.25M, preferably, 0.02 to 0.25M, in an acidic buffer having a pH of from about 2 to about 6.
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Hartman Anthony L.
Jones Christine R.
Klotz Janis L.
Ceperley Mary E.
Terlizzi Laura
Ventana Medical Systems, Inc.
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