Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or...
Patent
1997-06-26
1999-11-02
McKane, Joseph K.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
435183, 548413, 548520, 548521, 558 11, C07D207333
Patent
active
059767839
ABSTRACT:
Formation of an intramolecular cross-link in enzyme donor polypeptide fragments of .beta.-galactosidase, thereby forming a cyclic enzyme donor which is hindered from complementation with an enzyme acceptor fragment to form active of .beta.-galactosidase. The cyclic enzyme donor can be linearized by cleaving to restore complementation ability. Assays in which such cyclic enzyme donors are linearized by specific analytes are disclosed, as well as novel homobifunctional bis-maleimido cross-linking agents of the formula ##STR1## wherein R is hydroxy or acetate.
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Powell Michael J.
Tietze Lutz F.
Boehringer Mannheim Corporation
McKane Joseph K.
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