Biphasic shuttle vectors

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

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435 91, 435170, 4351723, 4352523, 435320, 435822, 4352521, 536 27, 935 6, 935 22, 935 60, 935 72, C12P 2100, C12P 1934, C12P 104, C12N 1500, C07H 1512

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049562800

ABSTRACT:
This invention provides a biphasic shuttle vector capable replication and expression of foreign genetic information in Escherichia coli and Agmenellum quadruplicatum comprising an E. coli replicon, an A. quadruplicatum replicon, at least two selectable markers and a promoter derived from A. quadruplicatum. The invention further provides an ectopic mutant of Agmenellum quadruplicatum PR-6 characterized by displaying less AquI interference with unmodified plasmids than the PR-6 strain.

REFERENCES:
Buzby et al., 1983 J. Bacteriol 154: 1446-1450.
Morrison et al. 1984 J. Bacteriol 159: 870-876.
Glass, Robert E. 1982 Gene Function Univ. Calif. Press Berkeley, pp. 364-365.
De Lorimier et al., 1984 PNAS 81: 7946-7950.
Mol. Gen. Genet. 199: 372-380, 1985. P. M. Andreoli. Versatile Escherichia Coli-Bacillus Shuttle Vectors Derived from Runaway Replication Plasmids Related to CloDF13.
Science 230: 805-807, 1985. J. S. Buzby et al., Expression of the Escherichia Coli lac Z Gene on a Plasmid Vector in a Cyanobacterium.
Gene 34: 363-366, 1985. M. Heusterpreute et al., Vectors with Restriction-Site Bands. III. Escherichia Coli-Saccharomyces Cerevisiae Shuttle Vectors.
Gene 39: 281-286, 1985. K. Miwa et al. Construction of Novel Shuttle Vectors and a Cosmid Vector for the Glutamic Acid-Producing Bacteria Brevibacterium lactofermentum and Corynebacterium glutamicum.
Appl. Environ. Microbiol. 50: 540-542, 1985., J. van der Vossan et al. Construction of Cloning, Promoter-Screening, and Terminator-Screening Shuttle Vectors for Bacillus Subtilis and Streptococcus Lactis.
Gene 30: 69-77, 1984. C. Morandi et al. Expression of Human Dihydrofolate Reductase cDNA and Its Induction by Chloramphenicol in Bacillus Subtilis.
Biotechnology 3: 69-72, 1985. L. Meile et al. Potential Shuttle Vectors Based on the Methanogen Plasmid pMEZ001.

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